胶原诱导性关节炎发生后不同时间大鼠骨骼的变化  

Bone changes at different time after the occurrence of collagen-induced arthritis in rats

作　　者：陈文双 许碧莲 林长缨 田佳 张新乐 CHEN Wen-Shuang;XU Bi-Lian;LIN Chang-Ying(Guangdong Medical University,Zhanjiang 524023,Guangdong,China)

机构地区：[1]广东医科大学,广东湛江524023 [2]广东医科大学附属医院

出　　处：《中国老年学杂志》2020年第24期5250-5254,共5页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(81373499)。

摘　　要：目的探讨胶原诱导性关节炎(CIA)发生后不同时间大鼠骨骼的变化。方法选用70只雌性SD大鼠随机选取24只为对照组,46只用于建立CIA模型。自初次免疫3 w后筛选出成功发生关节炎大鼠24只作为CIA组,然后4、8、12、16 w后每次随机处死6只取材。取踝关节进行组织病理学评分,取股骨进行骨密度、骨生物力学和Micro-CT的分析。结果与正常对照组比较,CIA组4、8、12、16 w大鼠股骨骨密度、骨生物力学参数和骨量均明显降低,而踝关节病理学评分明显增高(P<0.01)。结论CIA发生后4 w大鼠开始出现骨质疏松,而且随着CIA发生时间越长,大鼠出现的骨质疏松越严重。Objective To investigate the bone changes at different time after the occurrence of collagen-induced arthritis(CIA)in rats.Methods From 70 female rats,24 rats were randomly selected as control group,and 46 rats were used to establish the CIA model.Three weeks after first immunization,24 rats which were successfully infected with arthritis were selected as CIA group.6 rats were randomly killed on the 4 w,8 w,12 w and 16 w after CIA respectively.The ankles were collected for histopathological examination and the femurs were collected for bone mineral density(BMD),biomechanical properties and micro-CT examination.Results Compared with control group,the BMD,biomechanical properties and bone mass of femur were significantly decreased,while histopathologic score of ankle was significantly increased on the 4 w,8 w,12 w and 16 w after CIA.Conclusions Osteoporosis could be seen on the 4 w after CIA and osteoporosis could become more serious with prolonged onset of CIA.

关 键 词：类风湿关节炎 骨质疏松 胶原诱导性关节炎 MICRO-CT 骨生物力学 

分 类 号：R322.7[医药卫生—人体解剖和组织胚胎学]