表皮葡萄球菌小菌落突变株的分离与鉴定  

Isolation and identification of small colony mutants of Staphylococcus epidermidis

作　　者：申燕[1] 周波 罗婷婷[1] 熊元 SHEN Yan;ZHOU Bo;LUO Tingting;XIONG Yuan(Department of Laboratory Medicine,Chongqing Maternal and Child Health Hospital,Chongqing 401147,China)

机构地区：[1]重庆市妇幼保健院检验科,重庆401147

出　　处：《现代医药卫生》2020年第24期3911-3913,3917,共4页Journal of Modern Medicine & Health

基　　金：重庆市卫生健康委员会面上项目(2017MSXM107)。

摘　　要：目的对临床标本中分离出的1株疑似表皮葡萄球菌小菌落突变株(SCVs)进行鉴定和相关特征研究,为SCVs感染的诊断与治疗提供依据。方法从临床患者的标本中分离培养出1株疑似表皮葡萄球菌SCVs,通过菌落形态、生长曲线、生化鉴定、药敏实验、16S rRNA基因聚合酶链式反应(PCR)及测序鉴定、化学限定培养基补偿实验等方面来分析其特征。结果通过16S rRNA基因测序鉴定该株SCVs为表皮葡萄球菌。与野生株比较,该菌株在血平板上生长缓慢、菌落细小;在液体培养中生长速度缓慢。胸腺嘧啶、盐酸硫胺素、血红素、甲奈醌及CO 2环境对其都有补偿作用。结论已成功分离并鉴定1株表皮葡萄球菌SCVs,可为进一步开展表皮葡萄球菌SCVs相关疾病的研究提供生物学材料。Objective To identify and study related characteristics of a suspected small colony mutant of Staphylococcus epidermidis(SCVs)isolated from clinical specimens to provide evidence for the diagnosis and treatment of SCVs infection.Methods A suspected Staphylococcus epidermidis SCVs was isolated and cultured from clinical patient specimens.The characteristics were analyzed by colony morphology,growth curve,biochemical identification,drug sensitivity test,16S rRNA gene polymerase chain reaction(PCR)and sequencing identification,chemical limited medium compensation experiment.Results The SCVs were identified as Staphylococcus epidermidis through 16S rRNA gene sequencing.Compared with the wild strain,the strain grew slowly on blood plates and had small colonies;it grew slowly in liquid culture.Thymine,thiamine hydrochloride,heme,menaquinone and CO2 environment all had a compensatory effect.Conclusion A strain of Staphylococcus epidermidis SCVs has been successfully isolated and identified,which can provide biological materials for further research on Staphylococcus epidermidis SCVs-related diseases.

关 键 词：表皮葡萄球菌 小菌落突变株 分离 鉴定 CO 2环境 

分 类 号：R446.5[医药卫生—诊断学]