基于网络药理学探讨四妙丸治疗骨关节炎的作用机制  

Mechanism of Simiao Pills in Treatment of Osteoarthritis Based on Network Pharmacology

作　　者：吴越 刘维[1,2] WU Yue;LIU Wei(First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;Tianjin Key Laboratory of Traditional Chinese Medicine Formula and Syndrome Transformation Research,Tianjin 300193,China)

机构地区：[1]天津中医药大学第一附属医院,天津300193 [2]天津市中医方证转化研究重点实验室,天津300193

出　　处：《世界中医药》2020年第23期3604-3611,3616,共9页World Chinese Medicine

基　　金：国家自然科学基金项目(81673927,81273709);天津市科技计划项目(15ZXLCSY00020)。

摘　　要：目的:采用网络平台药理学的研究方法,对四妙丸(SMW)用于治疗骨关节炎(OA)的药理作用以及机制问题进行了研究和探讨。方法:主要通过在TCMSP网络平台对四妙丸进行活性药物成分的筛选、在DrugBank数据库的基础上进行了靶标的预测,应用Cytoscape 3.7.1软件构建了化合物-靶点数据分析网络;在TTD及DisGenet进行数据库的检索,获取了疾病的靶点;通过构建韦恩图,取得了疾病与活性药物作用靶点的交集,得到了四妙丸治疗OA的作用靶点;并对四妙丸进行了GO功能注释功能通路注释和KEGG通路富集分析;构建了四妙丸中药-成分-靶点-信号通路网络。结果:分析研究显示共挖掘到了四妙丸中64种共同作用成分及163个潜在共同作用靶点,OA疾病989个成分及相关共同作用靶点,得到四妙丸-疾病共同靶点18个,共同信号作用的靶点主要富集于34个生物过程和26条信号通路上。结论:四妙丸中药可能主要是通过调节PTGS2、TNF、AR等共同靶点,调控IL-17信号通路、TNF信号通路、松弛素、破骨细胞分化、松弛素等方式来抑制机体炎性反应,进一步调节机体免疫功能来治疗骨关节炎。Objective:To study the mechanism of Simiao Pills in the treatment of osteoarthritis(OA),based on network pharmacology.Methods:By screening the active ingredients of Simiao Pills on TCMSP platform,the target in the database of drugbank was predicted.Cytoscape 3.7.1 software was used to construct the compound-target data analysis network;the database was searched in TTD and DisGenet to obtain the target of the disease;the Venn diagram was constructed to obtain the intersection of the target of the disease and the active drug.The target of Simiao Pill in the treatment of OA was obtained;GO function annotation functional pathway annotation and KEGG pathway enrichment analysis were performed on Simiao Pill;a network of Simiao Pill Chinese medicine-component-target-signaling pathway was constructed.Results:The research showed that 64 components and 163 potential targets in Simiao Pills,989 related targets in OA disease,and 18 common targets in Simiao Pills disease were obtained,which were mainly concentrated in 34 biological processes and 26 signal pathways.Conclusion:Simiao Pills may be used for the treatment of osteoarthritis by regulating PTGS2,TNF,AR and other targets,regulating IL-17 signaling pathway,TNF signaling pathway,relaxin,osteoclast differentiation and other pathways,so as to inhibit inflammatory response etc.

关 键 词：四妙丸 网络药理学 骨关节炎 靶点 信号通路 

分 类 号：R285[医药卫生—中药学]