平喘汤对哮喘小鼠模型NLRP3炎症小体活化的影响  

Effects of Pingchuan Decoction on NLRP3 Inflammasome Activation Induced by Asthma in Mice

作　　者：昝杰彧 孙剑玥[1] 徐亚娜 付艳缇 解玉[1] ZAN Jieyu;SUN Jianyue;XU Yana;FU Yanti;XIE Yu(Department of Pediatrics,Putuo Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200062,China)

机构地区：[1]上海中医药大学附属普陀医院儿科,上海200062

出　　处：《世界中医药》2020年第23期3646-3652,共7页World Chinese Medicine

基　　金：上海市普陀区卫生系统自主创新科研资助项目(ptkwws201713)。

摘　　要：目的:探究平喘汤对小鼠支气管气道炎性反应的抑制作用,及其作用机制是否与NLRP3炎症小体活化的调控相关。方法:将6~8周龄C57BL/6雄性小鼠38只随机分为空白对照组、哮喘模型组、低剂量平喘汤组(15 g/kg)、中等剂量平喘汤组(30 g/kg)、高剂量平喘汤组(60 g/kg)及阳性药物(地塞米松)对照组。采用腹腔注射及滴鼻吸入卵蛋白致敏法制备小鼠支气管哮喘模型。除阳性对照组予地塞米松腹腔给药外,余各组均予对应药物灌胃,连续4周。末次给药后,观察小鼠一般情况,行为学评分(打喷嚏、抓鼻次数,呼吸急促程度),小鼠肺泡灌洗液中细胞总数、中性粒细胞、嗜酸性粒细胞、淋巴细胞及巨噬细胞计数,肺泡灌洗液炎性反应因子IL-1β、IL-6、TNF-α及MCP-1表达,肺组织NLRP3炎症小体及其下游靶向炎性反应因子IL-1β及IL-18表达情况。结果:平喘汤可有效减少哮喘小鼠行为学评分,降低肺泡灌洗液细胞总数、中性粒细胞、嗜酸性细胞、淋巴细胞及巨噬细胞计数,下调肺泡灌洗液炎性反应因子IL-1β、IL-6、TNF-α及MCP-1表达,抑制肺组织NLRP3炎症小体、IL-1β及IL-18 mRNA和蛋白表达情况。结论:平喘汤可通过抑制NLRP3炎症小体活化介导的气道炎性反应进而减轻哮喘呼吸道症状。Objective:To explore the inhibitory effect of Pingchuan Decoction on bronchial airway inflammation in mice,and whether its mechanism is related to the regulation of NLRP3 inflammasome activation.Methods:A total of 38 C57BL/6 male mice aged 6-8 weeks were randomly divided into a blank control group,an asthma model group,a low-dose Pingchuan Decoction group(15 g/kg),and a medium-dose Pingchuan Decoction group(30 g/kg),a high-dose Pingchuan Decoction group(60 g/kg)and a positive drug(dexamethasone)and a control group.A mouse bronchial asthma model was prepared by intraperitoneal injection and intranasal inhalation of egg protein sensitization method.Except for the positive control group who was given dexamethasone intraperitoneally,the other groups were given the corresponding drugs by gavage for 4 consecutive weeks.After the last administration,the general condition of the mice,behavioral scores(number of sneezing,scratching,and degree of shortness of breath),the total number of cells in the mouse alveolar lavage fluid,neutrophils,eosinophils,lymphocytes and Macrophage count,expression of inflammatory factors IL-1β,IL-6,TNF-αand MCP-1 in alveolar lavage fluid,expression of NLRP3 inflammasome and its downstream target inflammatory factors IL-1βand IL-18 in lung tissue were observed.Results:Pingchuan Decoction can effectively reduce the behavioral scores of asthmatic mice,reduce the total number of alveolar lavage fluid cells,neutrophils,eosinophils,lymphocytes and macrophages,and down-regulate the inflammatory factors IL-1βand IL in the alveolar lavage fluid-6,TNF-αand MCP-1 expression,inhibit the expression of NLRP3 inflammasome,IL-1βand IL-18 mRNA and protein in lung tissue.Conclusion:Pingchuan Decoction can reduce the respiratory tract symptoms of asthma by inhibiting the airway inflammation mediated by the activation of NLRP3 inflammasomes.

关 键 词：平喘汤 哮喘 气道炎性反应 NLRP3炎症小体 IL-1Β IL-18 

分 类 号：R242[医药卫生—中医临床基础] R285.5[医药卫生—中医学]