水痘减毒活疫苗初次免疫后11年免疫持久性研究  

Immune persistence of Live Attenuated Varicella Vaccine after 11 years of primary immunization

作　　者：黄莉荣[1] 金莎 耿钊 莫兆军[1] 陶航 HUANG Li-Rong;JIN-Sha;GENG Zhao;MO Zhao-Jun;TAO Hang(Center for Disease Control and Prevention of Guangxi,Nanning 530028,China)

机构地区：[1]广西壮族自治区疾病预防控制中心,南宁530028 [2]长春百克生物科技股份公司,长春130012

出　　处：《中国免疫学杂志》2020年第20期2558-2560,F0003,共4页Chinese Journal of Immunology

摘　　要：目的:观察水痘减毒活疫苗(VarV)受试者初次免疫后11年的抗体几何平均滴度(GMT)和阳性率,并评价其免疫持久性。方法:选择2006年9月于广西永福县开展的VarV随机、双盲Ⅲ期临床试验中接种1针剂国产VarV的受试者100名作为试验组,50名接种1针剂进口VarV(平行试验)的受试者作为对照组;采集初次免疫后11年的静脉血3.0 ml,膜抗原荧光抗体法检测血清中水痘病毒抗体,计算血清抗体阳性率、抗体GMT和不同中和抗体滴度人数的百分比,并与初次免疫后30 d抗体水平进行比较。结果:VarV初次免疫后11年,2组全人群、幼儿和儿童抗体GMT分别为37.01、42.31、28.21和22.63、22.83、21.93,抗体阳性率分别为98.00%、100.00%、93.94%和94.00%、92.31%、100.00%,中和抗体滴度≥4的人数占比分别为98.00%、100.00%、93.94%和94.00%、92.31%、100.00%;试验组幼儿的GMT、抗体阳性率及中和抗体滴度≥4的人群数占比明显高于对照组(P<0.05);2组全人群、幼儿及儿童的抗体GMT均明显低于初免后30 d(P<0.05),但抗体阳性率仍保持较高水平(≥92.31%)。结论:VarV免疫后11年仍具有较好的免疫原性,但抗体GMT水平随时间推移呈明显下降趋势。Objective:To observe geometric mean titer(GMT)and positive rate of varicella antibody after 11 years of subjects of primary immunization of freeze-dried varicella attenuated live vaccine(VarV),and its immune persistence was evaluated.Methods:100 subjects inoculated one dose of domestic VarV as experiment group and 50 subjects who inoculated one dose of imported VarV(parallel trial)as control group who were recruited from randomized,blind clinical trial phaseⅢconducted in Yongfu County,Guangxi province in September 2006.After 11 years of primary immunization,3.0 ml of venous blood was collected,varicella virus antibody in serum was detected by fluorescent antibody to membrane antigen method,positive rate of serum antibody was calculated,GMT of antibody and percentage of people with different neutralizing antibody titer were counted,which were compared with antibody level 30 d after primary immunization.Results:11 years after primary immunization,GMT of antibody in whole population,infant,and children groups of two groups were 37.01%,42.31%,28.21%and 22.63%,22.83%,21.93%,positive rates were 98.00%,100.00%,93.94%and 94.00%,92.31%,100.00%,percentage of neutralizing antibody titers≥4 was 98.00%,100.00%,93.94%and 94.00%,92.31%,100.00%,respectively;GMT,antibody positive rate and percentage of neutralizing antibody titer≥4 in whole population and children in trial group were significantly higher than those in control group(P<0.05);GMT of VZV antibody in whole population,infant,and children groups of two groups were significantly lower than that of 30 d after primary immunization(P<0.05),but antibody positive rate is still high(≥92.31%).Conclusion:VarV still had good immunogenicity after 11 years of primary immunization,but antibody GMT level decreased with time.

关 键 词：水痘减毒活疫苗 免疫持久性 几何平均滴度 抗体 阳性率 

分 类 号：R186[医药卫生—流行病学] R511.5[医药卫生—公共卫生与预防医学]