介绍一种外泌体负染色的改良方法  

An improved method for negat ive staining of exosomes was introduced

作　　者：金良韵[1] 姬曼[1] 孙竹林[1] 杨慧[1] 陈大兴[1] 赵君朋[1] JIN Liang-yun;JI Man;SUN Zhu-lin;YANG Hui;CHEN Da-xing;ZHAO Jun-peng(Capital Medical University Central Laboratory,Bei jing 100069,China)

机构地区：[1]首都医科大学,100069

出　　处：《首都食品与医药》2020年第22期91-92,共2页Capital Food Medicine

摘　　要：目的为在透射电镜下观察研究外泌体结构形态提供负染色方法.透射电镜是观察鉴定外泌体结构形态的重要研究手段.因常用负染色方法有很多种,包括醋酸双氧铀、磷钨酸、钼酸铵等染色对其外泌体负染色,且技术步骤也均有不同.方法本文介绍的方法是以传统醋酸双氧铀染色方法作为对比,介绍一种针对外泌体改良后的负染色方法并与之进行比较.结果与结论改良后的负染色方法外泌体的双层膜结构清晰,其杯托状结构清晰可见,且背景干净,反查效果佳.Objective To provide a negative staining method for the study of exosome structure under transmissione lectron.microscope.Transmission electron microscope(TEM)is an important research method to observe and identify the structure of exosomes.There are many kinds of negative staining methods,including hydrogen peroxide acetate,phosphotungstic acid、ammonium molybdate,etc.Methods The method introduced in this paper is to compare with the traditional method of hydrogen peroxide acetate,and introduce a negative staining method for exosomes.Results and Conc lusi on The modified staining method has clear rdouble membrane structure,clear cup-like structure,clean background and good anti-examination effect.

关 键 词：透射电镜技术 负染色 外泌体 样品制备 

分 类 号：Q813[生物学—生物工程]