支气管镜导引下圈套器电灼损伤在犬气管狭窄模型中的应用  

Application of bronchoscope-guided snare electrocautery injury of airway in establishing canine tracheal stenosis model

作　　者：赵纯 向述天[1] 苏伟[1] 曾俊仁[1] 徐松[1] 赵娟娟[1] 毛崇文[1] 代洪妍 ZHAO Chun;XIANG Shuntian;SU Wei;ZENG Junren;XU Song;ZHAO Juanjuan;MAO Chongwen;DAI Hongyan(Department of Radiology,Forth Affiliated Hospital of Kunming Medical University,Kunming,Yunnan Province 650021,China)

机构地区：[1]昆明医科大学第四附属医院放射科,650021

出　　处：《介入放射学杂志》2020年第11期1116-1119,共4页Journal of Interventional Radiology

基　　金：云南省科技厅-昆明医科大学联合专项基金项目(2017FE468-087)。

摘　　要：目的探讨纤维支气管镜导引下圈套器电灼犬气管建立气管狭窄模型的可行性、安全性和有效性。方法支气管镜导引下用电圈套器灼伤10只健康比格犬气管中段。术后观察犬气促、喘息等情况,每周行支气管镜、CT检查,计算狭窄程度。术后2、4周各处死1只犬,取气管组织行苏木精-伊红(HE)和Masson染色,观察组织炎症和纤维化程度。结果所有犬气管电灼伤术均获成功。术后14 d、19 d,所有犬均出现喘息和肋间隙凹陷。术后7 d、14 d、21 d、28 d气管狭窄程度中位数分别为26%、72%、86%、90%。组织病理学观察显示,术后14 d气管黏膜见重度炎症浸润,伴少量纤维组织生长;术后25 d炎症浸润以淋巴细胞为主,见多量纤维化。结论支气管镜导引下圈套器电灼法建立犬气管狭窄模型,具有操作简便、快速,安全,可重复性强,临床相似度高等优点。Objective To discuss the feasibility,safety and effectiveness of fiberoptic bronchoscopeguided snare electrocautery injury of airway in establishing canine tracheal stenosis model.Methods Bronchoscope-guided snare electrocautery injury of tracheal middle segment was performed in 10 healthy beagle dogs.The postoperative conditions such as shortness of breath and wheezing were recorded.The fiberoptic bronchoscopy and CT examinations were performed every week to calculate the stenosis degree.One dog was sacrificed each time at postoperative 2 and 4 weeks,the tracheal tissue was collected,stained with hematoxylin-eosin(HE)and Masson,and sent for pathological examination to observe the tissue inflammation and fibrosis.Results Successful bronchoscope-guided snare electrocautery injury of trachea was accomplished in all the experimental dogs.Fourteen and 19 days after treatment,all dogs developed wheezing and intercostal depression.The median tracheal stenosis degrees at 1,2,3 and 4 weeks after treatment were 26%,72%,86%and 90%,respectively.Histopathological examination showed that severe inflammation of tracheal mucosa was observed at 14 days after treatment,and large amount of fibrosis with mainly lymphocytic infiltration was seen at 25 days after treatment.Conclusion For the establishment of tracheal stenosis model in experimental dogs,fiberoptic bronchoscope-guided snare electrocautery injury method is technically easy and fast,and clinically safe,besides,it can be repeatedly adopted.Moreover,the pathological characteristics of this model are highly similar to the clinical features of tracheal stenosis.

关 键 词：气管狭窄 动物模型 电灼 圈套器 

分 类 号：R562.1[医药卫生—呼吸系统]