低硒条件下T-2毒素对大鼠关节软骨及骺板软骨肝细胞生长因子及其受体表达的影响  

Effects of T-2 toxin on expression of hepatocyte growth factor and its receptor in articular cartilage and epiphyseal cartilage of rats under selenium deficiency

作　　者：冯依萍 王文君 刘毅楠 何颖 李正正 方倩 张迎 王会 张萌 刘悦 廖昱程 陈静宏 Feng Yiping;Wang Wenjun;Liu Yinan;He Ying;Li Zhengzheng;Fang Qian;Zhang Ying;Wang Hui;Zhang Meng;Liu Yue;Liao Yucheng;Chen Jinghong(Institute of Endemic Diseases,School of Public Health,Health Science Center of Xi'an Jiaotong University,Xi'an 710061,China)

机构地区：[1]西安交通大学医学部公共卫生学院地方病研究所,710061

出　　处：《中华地方病学杂志》2020年第12期881-886,共6页Chinese Journal of Endemiology

基　　金：国家自然科学基金(81573102、81872565、81273006)。

摘　　要：目的观察低硒条件下T-2毒素对大鼠关节软骨及骺板软骨肝细胞生长因子(HGF)、HGF受体(C-Met)表达的影响。方法选择24只健康雄性SD大鼠,体质量为60~80 g,按体质量采用随机数字表法分为常规饲料组(硒含量为101.5μg/kg)和低硒饲料组(硒含量为1.1μg/kg),每组12只。喂养30 d后,将常规饲料组分为常规组和T-2毒素组(100μg·kg^-1·d^-1),低硒饲料组分为低硒组和低硒+T-2毒素组(100μg·kg^-1·d^-1),每组6只。继续喂养30 d后处死大鼠,取膝关节软骨组织,采用HE染色光镜下观察膝关节软骨及骺板软骨形态学改变;免疫组织化学法检测膝关节软骨及骺板软骨HGF和C-Met表达情况,计算HGF和C-Met阳性表达率。结果光镜下,低硒+T-2毒素组关节软骨及骺板软骨细胞排列稀疏,深层可见坏死无结构区,区域内软骨细胞细胞外基质降解而淡染,附近可见增生的肉芽组织。低硒、T-2毒素、低硒+T-2毒素组大鼠关节软骨及骺板软骨HGF阳性表达率[(21.97±6.90)%、(49.41±8.24)%、(76.39±5.88)%,(23.36±12.49)%、(58.43±14.48)%、(66.59±10.83)%]高于常规组[(9.13±6.01)%、(11.14±4.67)%,P均<0.05]。低硒、T-2毒素、低硒+T-2毒素组关节软骨及骺板软骨C-Met阳性表达率[(25.34±7.53)%、(58.21±12.54)%、(81.46±7.89)%,(35.21±4.71)%、(40.84±2.03)%、(49.41±6.29)%]高于常规组[(11.21±5.11)%、(12.12±4.71)%,P均<0.05]。结论低硒条件下T-2毒素对大鼠关节软骨及骺板软骨HGF、C-Met表达有影响。Objective To observe the effects of T-2 toxin on expression of hepatocyte growth factor(HGF)and HGF receptor(C-Met)in articular cartilage and epiphyseal cartilage of rats under low selenium condition.Methods Twenty-four healthy male SD rats weighted 60-80 g were randomly divided into conventional diet group(selenium content of 101.5μg/kg)and low-selenium diet group(selenium content of 1.1μg/kg),with 12 rats in each group.After 30 days of feeding,the conventional diet group was further divided into conventional group and T-2 toxin group(100μg·kg^-1·d^-1),and the low-selenium diet group was further divided into low-selenium group and low-selenium+T-2 toxin group(100μg·kg^-1·d^-1),with 6 rats in each group.After 30 days of feeding,the rats were sacrificed and the cartilage of knee joint was taken,the morphological changes of knee articular cartilage and epiphyseal cartilage were observed by HE staining under light microscope.Immunohistochemical method was used to detect the expression of HGF and C-Met in knee articular cartilage and epiphyseal cartilage,and positive expression rates of HGF and C-Met were calculated.Results Under light microscope,chondrocytes of articular cartilage and epiphyseal cartilage in low-selenium+T-2 toxin group were sparse,and the necrosis and structural area were found in the deep layer,and the extracellular matrix of chondrocytes in the region was degraded and light stained,and proliferating granulation tissue was visible nearby.The positive expression rates of HGF in articular cartilage[(21.97±6.90)%,(49.41±8.24)%,(76.39±5.88)%]and epiphyseal cartilage[(23.36±12.49)%,(58.43±14.48)%,(66.59±10.83)%]of rats in low-selenium,T-2 toxin and low-selenium+T-2 toxin groups were higher than those in conventional group[(9.13±6.01)%,(11.14±4.67)%,P<0.05].The positive expression rates of C-Met in articular cartilage[(25.34±7.53)%,(58.21±12.54)%,(81.46±7.89)%]and epiphyseal cartilage[(35.21±4.71)%,(40.84±2.03)%,(49.41±6.29)%]of rats in low-selenium,T-2 toxin and low-selenium+T-2 t

关 键 词：硒 T-2毒素 大骨节病 肝细胞生长因子 原癌基因蛋白质C-MET 

分 类 号：R684.1[医药卫生—骨科学]