隐丹参酮抑制模型兔耳增生性瘢痕的作用及机制  

Effect of cryptotanshinone on hypertrophic scar of rabbit ear and its related mechanism

作　　者：杨莉 李雪莉 宋静卉 禹卉千 王伟霞 Yang Li;Li Xueli;Song Jinghui;Yu Huiqian;Wang Weixia(Department of Dermatology,Henan Provincial People’s Hospital,Zhengzhou University People’s Hospital,Zhengzhou 450003,Henan Province,China)

机构地区：[1]河南省人民医院皮肤科,郑州大学人民医院,河南省郑州市450003

出　　处：《中国组织工程研究》2021年第20期3150-3155,共6页Chinese Journal of Tissue Engineering Research

基　　金：河南省医学教育研究项目(Wjlx2018086),项目负责人:李雪莉。

摘　　要：背景:研究表明隐丹参酮对增生性瘢痕组织增生具有一定的抑制作用,但具体机制尚不明确。目的:探讨隐丹参酮对兔耳增生性瘢痕组织的抑制作用及其对转化生长因子β1/Smads信号通路的影响。方法:建立增生性瘢痕兔耳模型,随机分为模型组、隐丹参酮27 mg/L组和隐丹参酮81 mg/L组,3组于建模后21 d分别局部注射生理盐水、27 mg/L及81 mg/L隐丹参酮,1次/d,共7 d;同时以正常兔为对照组。给药结束后测量瘢痕指数,取材后采用苏木精-伊红染色和Masson染色进行病理学分析,免疫组化法检测α-平滑肌肌动蛋白表达,碱水解法检测羟脯氨酸含量,免疫印迹法检测Ⅰ,Ⅲ型胶原及转化生长因子β1/Smads信号通路相关蛋白表达水平。结果与结论:①与对照组比较,模型组瘢痕指数、成纤维细胞及胶原纤维数量明显升高,瘢痕组织中α-平滑肌肌动蛋白水平、羟脯氨酸含量及Ⅰ,Ⅲ型胶原、转化生长因子β1、p-Smad2、p-Smad3、Smad4的蛋白表达水平均明显升高(P<0.05);②与模型组比较,隐丹参酮27 mg/L组和隐丹参酮81 mg/L组瘢痕指数、成纤维细胞及胶原纤维数量明显降低,瘢痕组织中α-平滑肌肌动蛋白水平、羟脯氨酸含量及Ⅰ,Ⅲ型胶原、转化生长因子β1、p-Smad2、p-Smad3、Smad4的蛋白表达水平均明显降低(P<0.05),且隐丹参酮81 mg/L组变化更明显(P<0.05);③提示隐丹参酮对兔耳增生性瘢痕的形成具有一定的抑制作用,其机制可能与转化生长因子β1/Smads信号通路有关。BACKGROUND:Cryptotanshinone(CTS)has been shown to have a certain inhibitory effect on hyperplastic scar hyperplasia,but the specific mechanism is still unclear.OBJECTIVE:To investigate the inhibitory effect of CTS on hypertrophic scar of the rabbit ear and its effect on transforming growth factor-β1/Smads signaling pathway.METHODS:The rabbit ear model of hypertrophic scar was established and then rabbit models were randomly divided into model group,low and high-dose CTS groups,followed by local injection of normal saline,27 and 81 mg/L CTS respectively beginning at 21 days after modeling,once a day,for 7 days.Meanwhile,normal rabbits were used as the control group.Scar index was measured after administration.Pathological analysis was carried out by hematoxylin-eosin staining and Masson staining after sampling.The expression ofα-smooth muscle actin was detected by immunohistochemistry.Hydroxyproline content was determined by alkaline hydrolysis.The expression of type I collagen,type III collagen and transforming growth factor-β1/Smads signaling pathway related proteins were detected by western blot.RESULTS AND CONCLUSION:Compared with the control group,the scar index,the number of fibroblasts and collagen fibers were significantly increased,the levels ofα-smooth muscle actin and hydroxyproline in scar tissue,and the protein levels of type I collagen,type III collagen,transforming growth factor-β1,p-Smad2,p-Smad3 and Smad4 were significantly increased in the model group(P<0.05).Compared with the model group,the scar index,the number of fibroblasts and collagen fibers were significantly decreased,the levels ofα-smooth muscle actin and hydroxyproline in scar tissue,and the protein levels of type I collagen,type III collagen,transforming growth factor-β1,p-Smad2,p-Smad3 and Smad4 were significantly decreased in the low and high-dose CTS groups(P<0.05).The above-mentioned parameters changed more significantly in the high-dose CTS group compared to the low-dose CTS group(P<0.05).To conclude,CTS has a certain inhi

关 键 词：隐丹参酮 增生性瘢痕 胶原 转化生长因子Β1 SMADS 通路 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]