丛枝菌根真菌和根瘤菌互作对苜蓿根际土壤细菌群落结构的影响及PICRUSt功能预测分析  

Effect of interaction between arbuscular mycorrhizal fungi and Rhizobium on Medicago sativa rhizosphere soil bacterial community structure and PICRUSt functional prediction

作　　者：杨盼 翟亚萍 赵祥 王绍明[1] 刘红玲[2] 张霞[1] YANG Pan;ZHAI Ya-Ping;ZHAO Xiang;WANG Shao-Ming;LIU Hong-Ling;ZHANG Xia(College of Life Sciences,Shihezi University,Shihezi,Xinjiang 832000,China;Chengdu Normal University,Chengdu,Sichuan 611130,China)

机构地区：[1]石河子大学生命科学学院,新疆石河子832000 [2]成都师范学院,四川成都611130

出　　处：《微生物学通报》2020年第11期3868-3879,共12页Microbiology China

基　　金：国家自然科学基金(31560656,31871568)。

摘　　要：【背景】紫花苜蓿是优良的豆科牧草,可以与丛枝菌根(Arbuscular mycorrhizae,AM)真菌和根瘤菌形成共生关系,接种AM真菌和根瘤菌可以促进土壤氮、磷循环以及提高苜蓿产量。【目的】探究接种AM真菌和根瘤菌对苜蓿根际细菌群落结构和功能的影响。【方法】采集6个不同处理组苜蓿根际、非根际土壤样品,基于细菌16S rRNA基因V3-V4区进行高通量测序,分析比较不同处理组苜蓿根际、非根际土壤中细菌群落分布的规律,并采用PICRUSt软件对不同处理组间菌群功能进行预测。【结果】36个土壤样品中共检测到3849个OTU,分属于50门59纲132目249科595属398种。其中主要的优势菌门为Proteobacteria(52.81%-81.46%)、Bacteroidetes(7.83%-19.68%)及Actinobacteria(2.21%-16.4%)。与不接种相比,接种根内球囊霉和摩西球囊霉分别提高了Gammaproteobacteria和Bacteroidia有益菌的丰度,接种根瘤菌提高了固氮菌(Alphaproteobacteria)的丰度。PICRUSt功能预测表明,细菌菌群共有35个子功能,菌群功能丰富,代谢为最主要的功能,并且接种根瘤菌可增加氨基酸代谢,从而有利于植株N素循环,而接种AM真菌可能对于N循环有一定的抑制作用,相比于单接种AM真菌,双接种AM真菌和根瘤菌处理组碳水化合物代谢更强,从而更有益于植株的氮、磷循环。【结论】接种AM真菌和根瘤菌可分别提高苜蓿根系与氮、磷循环有关的不同有益菌的丰度,从而更有益于植株的氮、磷循环,该结果为提高植株养分吸收、提高苜蓿产量以及菌肥开发利用提供了科学依据。[Background]Medicago sativa is excellent leguminous forage that can reciprocate with arbuscular mycorrhizal(AM)fungi and rhizobium.Inoculation of AM fungi and rhizobium can promote soil nitrogen and phosphorus cycling and increase alfalfa yield.[Objective]To study the effects of AM fungi and Rhizobium on the structure and function of rhizosphere bacterial community.[Methods]Rhizosphere and non-rhizosphere soil samples of six different treatment groups were collected,based on the high-throughput sequencing technology of bacterial 16 S rRNA gene V3-V4 region.The bacterial diversity and community distribution in the rhizosphere and non-rhizosphere soils of different inoculation treatment groups were analyzed and compared.PICRUSt software was applied to predict the function of bacterial microbiota between different treatment groups.[Results]A total of 3849 OTU were obtained from 36 soil samples,belonging to 398 species of 50 divisions,59 classes,132 orders,249 families and 595 genera.The main dominant bacteria were Proteobacteria(66.79%,52.81%-81.46%),Bacteroidetes(7.83%-19.68%)and Actinobacteria(2.21%-16.40%).Compared with no inoculation,the inoculation treatment of Glomus intraradices and G.mosseae increased the beneficial bacteria of Gammaproteobacteria and Bacteroidia,inoculation with Rhizobium increased the abundance of the nitrogen-fixing bacteria Alphaproteobacteria.PICRUSt function prediction analysis indicateed that the bacterial microbiota had a total of 35 sub-functions.Inoculation with rhizobium can increase amino acid metabolism,which is conducive to plant N-nutrient cycle,and inoculation with AM fungi may have a certain inhibitory effect on N-cycle.Compared with single inoculation with AM fungi,double inoculation with AM fungi and rhizobium treatment group had stronger carbohydrate metabolism,which was more beneficial to plant nitrogen and phosphorus cycle.[Conclusion]The inoculation with AM fungi and rhizobium can increase the abundance of different beneficial bacteria related to nitrogen and phosphoru

关 键 词：苜蓿 高通量测序 细菌多样性 细菌群落结构 细菌功能预测 

分 类 号：S154.3[农业科学—土壤学] S541.9[农业科学—农业基础科学]