二仙汤对去卵巢大鼠心肌微血管及血液流变学的影响  

Effect of Erxian Decoction on Myocardial Microvessels and Hemorheology in Ovariectomized Rats

作　　者：姜燕华 王昱涵 刘燕君 向丽华[1] 张治国[1] 陈彦静[1] JIANG Yan-hua;WANG Yu-han;LIU Yan-jun;XIANG Li-hua;ZHANG Zhi-guo;CHEN Yan-jing(Institute of Basic Theory of Chinese Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院中医基础理论研究所,北京100700

出　　处：《中国实验方剂学杂志》2020年第24期59-67,共9页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中央级公益性科研院所基本科研业务费专项(YZ-1884);中国中医科学院中医药“一带一路”合作专项(GH2017-02-03)。

摘　　要：目的:观察去卵巢大鼠心肌微血管密度、微血管内皮细胞形态及血液流变学的变化并探讨二仙汤的干预作用。方法:选用健康10周龄雌性SPF级SD大鼠32只,随机分为假手术组、模型组、雌激素组(戊酸雌二醇,0.18 mg·kg^-1),二仙汤组(9 g·kg^-1)。去卵巢后2周开始灌胃给药,1次/d,连续16周。二仙汤组及雌激素组分别给予二仙汤或戊酸雌二醇灌胃,假手术组和模型组灌胃等体积纯净水。给药16周末,无创超声心动图(UCG)检测心功能;CD34免疫荧光染色法检测心肌微血管密度;透射电镜观测心肌微血管超微结构;放射免疫分析法检测血浆雌二醇(E2)含量;酶联免疫吸附测定(ELISA)检测血浆内皮素-1(ET-1),前列环素I2(PGI2),血栓素A2(TXA2),血管内皮生长因子(VEGF)和血管性血友病因子(vWF)水平;凝固法检测凝血四项;血液流变学检测全血黏度和血浆黏度。结果:与假手术组比较,模型组左室射血分数(EF)显著降低(P<0.01),左室短轴缩短率(FS)显著降低(P<0.01),左室收缩末期容积(LVVols)显著升高(P<0.01);心肌微血管密度显著减少(P<0.01);心肌微血管内皮细胞肿胀明显,胞浆空化;血浆E2含量显著降低(P<0.01);ET-1,VEGF,vWF含量显著升高(P<0.01),前列环素I2/血栓素A2(PGI2/TXA2)显著降低(P<0.01);血浆活化部分凝血酶原时间(APTT)显著降低(P<0.01),纤维蛋白原(FIB)含量显著升高(P<0.01);全血黏度、血浆黏度、卡松黏度显著升高(P<0.01),全血高、低切指数和红细胞聚集指数明显升高(P<0.05)。与模型组比较,二仙汤组大鼠EF,FS明显升高(P<0.05),LVVols明显降低(P<0.05);微血管密度显著增多(P<0.01);心肌微血管内皮细胞水肿改善,运输小泡清晰可见;血浆E2显著升高(P<0.01);ET-1,VEGF显著降低(P<0.01),PGI2/TXA2显著升高(P<0.01);APTT显著升高(P<0.01);全血黏度、全血高切相对指数、红细胞聚集指数明显降低(P<0.05),卡松黏度、血浆黏度显著降低(P<0.01)。结论:二仙�Objective: To observe the changes of myocardial microvessel density,microvascular endothelial cell morphology and hemorheology in ovariectomized rats and explore the interventional effects of Erxian decoction. Method: Thirty-two healthy 10 week-old female SPF SD rats were randomly divided into sham operation group,model group,estrogen group(estradiol valerate,0.18 mg·kg^-1)and Erxian decoction group(9 g·kg^-1). The rats were intragastrically administered 2 weeks after ovariectomy,once a day for 16 weeks. Sham operation groups and model groups were given equal volumes of purified water. After 16 weeks of administration,the cardiac function was measured by noninvasive ultrasound cardiogram(UCG),CD34 in the myocardial tissue was tested by immunofluorescence staining to measure the microvessel density, the morphological structure of microvessels of myocardial tissue were detected by transmission electron microscope,the levels of estrogen(E2)in rat plasma were detected by radioimmunoassay,the levels of endothelin-1(ET-1),prostacyclin I2(PGI2),thromboxane A2(TXA2),endothelial growth factor(VEGF),and von Willebrand Factor(vWF)in rat plasma were detected by enzyme-linked immuno sorbent assay(ELISA),four items of coagulation was detected by blood coagulation analyzer,whole blood viscosity and plasma viscosity were detected by hemorheology. Result: Compared with sham operation group,the ejection fraction(EF)decreased(P<0.01),the left ventricular short axis shortening rate(FS)decreased(P<0.01),and the left ventricular end systolic volume(LVVols)increased(P<0.01),myocardial microvessel density significantly reduced(P<0.01),the endothelial cells were swollen and the cytoplasm was cavitation,E2 in rat plasma decreased(P<0.01),ET-1,VEGF,vWF increased(P<0.01),prostacyclin I2/thromboxane A2(PGI2/TXA2)decreased(P<0.01),plasma activated partial prothrombin time(APTT)decreased(P<0.01),fibrinogen(FIB)increased(P<0.01),whole blood viscosity,plasma viscosity,and cassone viscosity increased(P<0.01),whole blood high-cut,lowcut index,an

关 键 词：去卵巢大鼠 心功能 心肌微血管 血液流变学 二仙汤 

分 类 号：R2-0[医药卫生—中医学] R289