铁皮石斛无菌纳米粉抗慢性肝损伤及对肝组织超微结构的影响  

Effect of Aseptic Dendrobium Officinale Nanoparticles on Chronic Liver Injury and Ultrastructure of Liver Tissue

作　　者：徐博 吴畏难[2] 沈楠 安英 XU Bo;WU Weinan;SHEN Nan;AN Ying(Basic Medical College,Jilin Medical University,Jilin 132013,China;Department of General Surgery,Central Hospital of Jilin City,Jilin 132011,China)

机构地区：[1]吉林医药学院基础医学院,吉林132013 [2]吉林市中心医院普通外科,吉林132011

出　　处：《医药导报》2021年第1期35-38,共4页Herald of Medicine

基　　金：吉林省卫生计生科技创新项目(2017ZC035);吉林省教育厅“十三五”科学技术项目(JJKH20180830KJ);吉林市科技创新发展计划项目(20163048)。

摘　　要：目的探讨铁皮石斛无菌纳米粉(DON)的保肝作用及其相关机制。方法建立四氯化碳(CCl4)慢性肝损伤小鼠模型,同时以受试药物灌胃干预,持续8周。昆明种小鼠随机分为正常对照组、模型对照组、DON组以及铁皮石斛普通粉(DOP)组。检测各组小鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、乳酸脱氢酶(LDH)和过氧化氢酶(CAT)活性;酶联免疫吸附测定(ELISA)检测血清中肿瘤坏死因子-α(TNF-α)和白细胞介素(IL)-6的表达;免疫组织化学法染色检测IL-1β的表达分布情况;苏木精-伊红(HE)染色行小鼠肝脏病理组织学观察;透射电镜观察肝组织超微结构变化。结果与模型对照组比较,DON和DOP干预后,血清ALT、AST、LDH水平均显著下降,CAT活性增强,TNF-α和IL-6的表达下调;免疫组化法显示,肝脏IL-1β的表达被显著抑制;肝脏病理组织学及肝细胞超微结构均有不同程度改善。与DOP组比较,DON组各项指标改善作用更显著。结论DON对慢性肝损伤具有更明显的保肝作用,其机制可能是DOP经过粒径改造后,更易促进机体抗氧化活性,抑制CCl4诱导的氧化应激,并具有抗炎作用。Objective To investigate the protective effect of aseptic dendrobium officinale nanoparticles(DON)on liver and its related mechanism.Methods The chronic liver injury mouse model of CCl4 was established,at the same time,the intervention was performed by intragastric administration of tested drugs for 8 weeks.Mice were randomly divided into normal control group,model control group,DON group and dendrobium officinale powders(DOP)group.The activity levels of glutamic oxalacetic transaminase(AST),glutamic pyruvic transaminase(ALT),lactate dehydrogenase(LDH)and catalase(CAT)in plasma of mice were assayed and compared.The levels of TNF-αand IL-6 were detected by ELISA.The expression of IL-1βin liver was observed by the immunohistochemical method.The pathology examination of the mice’s liver tissues was performed.Ultrastructural changes of liver tissues were observed by transmission electron microscopy.Results Comparing with the model control group,DON and DOP could reduce the activity of serum ALT,AST and LDH,and elevate serum CAT activity.The expression of TNF-αand IL-6 were down-regulated.They could decrease the level of IL-1βprotein expression which was tested by the immunohistochemical method.Liver histopathology and ultrastructure of hepatocytes were improved to some extent.Compared with the DOP group,the improvement effect of the above indicators was more significant in the DON group.Conclusion DON has a more obvious liver protection effect on chronic liver injury.The mechanism may be that dendrobium officinale nanoparticles are more likely to promote the antioxidant activity,inhibit the oxidative stress induced by CCl4 and the anti-inflammatory effect after particle size modification.

关 键 词：铁皮石斛 无菌纳米粉 肝损伤 保护作用 

分 类 号：R975.5[医药卫生—药品] R965[医药卫生—药学]