成虫玻璃管药膜法测定柑橘木虱田间种群对6种杀虫剂的敏感性  

Sensitivity of field populations of Diaphorina citri(Kuwayama) adults to six insecticides using residual film in glass tubes

作　　者：崔丽[1] 夏长秀 王立 王芹芹[1] 黄伟玲 袁会珠[1] 芮昌辉[1] CUI Li;XIA Changxiu;WANG Li;WANG Qinqin;HUANG Weiling;YUAN Huizhu;RUI Changhui(Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Key Laboratory of Integrated Pest Management in Crops,Ministry of Agriculture and Rural Affairs,Beijing 100193,China;Citrus Science Institute of Ganzhou,Jiangxi Province,Ganzhou 341401,Jiangxi Province,China)

机构地区：[1]中国农业科学院植物保护研究所,农业农村部有害生物综合防治重点实验室,北京100193 [2]江西省赣州市柑桔科学研究所,江西赣州341401

出　　处：《农药学学报》2020年第6期1094-1098,共5页Chinese Journal of Pesticide Science

基　　金：国家重点研发计划项目(2018YFD0201502)。

摘　　要：建立了采用玻璃管药膜法检测柑橘木虱成虫对杀虫剂敏感性的测定方法,并采用该方法检测了4个柑橘木虱田间种群对4类6种不同类型杀虫剂的敏感性。结果表明:柑橘木虱对噻虫啉、噻虫嗪和毒死蜱的抗性在0.57~3.07倍之间,尚处于敏感状态;但广西灵川种群对吡虫啉产生了18.90倍抗性,已处于中等抗性水平,同时,灵川种群对高效氯氟氰菊酯产生了9.72倍抗性,而且,广西灵川和广西永福种群已对新型杀虫剂氟啶虫胺腈产生了低水平抗性,抗性达5倍以上。测定结果与文献报道的田间药效试验结果具有良好的一致性。本研究可为柑橘木虱毒力测定方法的标准化和更科学有效地开展柑橘木虱对杀虫剂敏感性动态监测提供科学依据。A new bioassay method of residual film in glass tube was developed for the study of Diaphorina citri(Kuwayama) adult. The sensitivity of 4 field populations of D. citri to 6 insecticides was evaluated. The results indicated that the D. citri was susceptible to thiacloprid, thiamethoxam and chlorpyrifos with the resistance ratios in the range of(0.57-3.07)-fold. However, Lingchuan population in Guangxi Province developed moderate level of resistance to imidacloprid, with the resistance ratio of18.90-fold. Meanwhile, Lingchuan population developed 9.72-fold resistance to lambda-cyhalothrin.Moreover, Lingchuan and Yongfu populations in Guangxi Province had developed low resistance levels to the novel insecticide sulfoxaflor, and the resistance ratios were over 5-fold. These results were in accordance with the reported documents about the control efficacy in field. It is helpful to set up a standard method to monitor the sensitivity dynamic of D. citri.

关 键 词：玻璃管药膜法 柑橘木虱 田间种群 杀虫剂 敏感性 

分 类 号：S481.4[农业科学—农药学] TQ450.1[农业科学—植物保护]