真空低温纳米生物保存技术  

Vacuum and Nano Cryopreservation Technology

作　　者：冯海靠 张淼 顾宁 Feng Haikao;Zhang Miao;Gu Ning(Jiangsu Key Laboratory of Biomaterials and Devices School of Biological Science and Medical Engineering Southeast University,Nanjing 210009,China)

机构地区：[1]东南大学生物科学与医学工程学院江苏省生物材料与器件重点实验室,南京210009

出　　处：《真空科学与技术学报》2020年第11期1003-1011,共9页Chinese Journal of Vacuum Science and Technology

基　　金：国家重大研发计划(2017YFA0104302);国家自然科学基金创新研究群体(61821002);国家自然科学基金重点项目(51832001)。

摘　　要：低温保存技术能够延长生物材料的保存时间,在生物医学领域有着广泛应用。经过数十年的发展,已经成功实现了细胞、胚胎等小尺寸生物材料的低温保存,但对器官等复杂组织而言,传统低温保存技术仍然面临许多挑战。真空及纳米技术的应用有望解决这些难题。利用真空及纳米技术对冰晶的成核与结晶过程进行调控,能够提升过冷系统的稳定性,优化大体积玻璃态生物材料的复温过程,从而提升器官等复杂组织的低温保存效果。本文将聚焦于真空及纳米技术在过冷保存及无冰晶的玻璃化保存中的应用,分别从基本原理、研究进展、发展趋势等方面展开论述。The flow field,around the plasma synthetic jet at different flight-altitudes,was experimentally simulated with the lab-built test platform,comprising the pressure generator,plasma jet actuator,sensors and control units.The influence of the low atmospheric pressure on the plasma jet velocity was investigated.The results show that the low atmospheric pressure has a major impact.To be specific,as the pressure decreased(or the flight-altitude increased),the peak jet-velocity was increased,instead of being decreased;in 0~10,000 m range,the peak jet velocity linearly increased,at an increase-rate of about 15%to 25%for every 1,000 m height increase;at 10,000 m,the peak jet-velocity was two times as fast as that at the ground.As expected,the low atmospheric pressure little affected the performance and operation conditions of the plasma-jet actuator,including the loading voltage amplitude,frequency and duty cycle.

关 键 词：真空技术 纳米技术 低温保存 过冷 玻璃化 

分 类 号：R318[医药卫生—生物医学工程]