化学发光法微阵列蛋白芯片临床应用专家共识  

Expert consensus on clinical application of protein microarrays by chemiluminescence

作　　者：中国医学装备协会检验医学分会 徐英春 杜娟[2] 李金明 李永哲[2] 童明庆[4] 许斌[5] Laboratory Medicine Branch of China Medical Equipment Association

机构地区：[1]不详 [2]中国医学科学院北京协和医院检验科 [3]国家卫生健康委员会临床检验中心临床免疫室 [4]江苏省人民医院检验科 [5]江苏省临床检验中心

出　　处：《中华检验医学杂志》2020年第11期1075-1079,共5页Chinese Journal of Laboratory Medicine

摘　　要：微阵列蛋白芯片是研究蛋白质与蛋白质相互作用和识别疾病相关自身抗原的平台,也可以高通量的形式检测抗原与抗体之间免疫结合反应。该方法是一种微型化的固相免疫检测方法,在肿瘤、感染性疾病、自身免疫性疾病及变态反应性疾病的筛查、辅助诊断和治疗监测中逐渐得到临床认可。该共识对以平面基质为载体,采用化学发光法检测的蛋白芯片的临床应用进行规范化和标准化。Protein microarrays are platforms for studying protein-protein interactions and identifying disease-related self-antigens/autoantigens,which elicit an immune response in a high-throughput format.It is a miniaturized solid-phase immunoassay method,which has been gradually recognized because of its clinical practice in disease screening,auxiliary diagnosis and treatment monitoring of tumors,infectious diseases,autoimmune diseases and allergic diseases.This consensus has standardized and normalized the clinical application of protein chip with plane matrix as carrier and utilizing chemiluminescent technique.

关 键 词：蛋白质阵列分析 微阵列蛋白芯片 固相免疫 高通量 化学发光法 

分 类 号：R446[医药卫生—诊断学]