三子养亲汤对支气管哮喘模型大鼠尿皮质素及肺表面活性蛋白-D表达的影响  

Effect of Sanzi Yangqin Decoction on urine corticosteroids and pulmonary surfactant protein-D expression in bronchial asthma rats

作　　者：余洪刚 付大海 宋超 唐俊峰[2] 马静维 YU Honggang;FU Dahai;SONG Chao;TANG Junfeng;MA Jingwei(Department of Lung Diseases,Xi'an Hospital of Traditional Chinese Medicine,Xi'an 710012,China;Laboratory of Traditional Chinese Medicine Pharmacology,Shaanxi University of Traditional Chinese Medicine,Xianyang 712000,China;Department of Respiratory Medicine,Baoji Hospital of Traditional Chinese Medicine,Baoji 712000,China)

机构地区：[1]西安市中医医院肺病科,陕西西安710012 [2]陕西中医药大学中药药理学研究室,陕西咸阳712000 [3]宝鸡市中医医院呼吸内科,陕西宝鸡712000

出　　处：《药物评价研究》2020年第11期2187-2192,共6页Drug Evaluation Research

基　　金：西安市科技计划项目(201805103YX11SF37)。

摘　　要：目的探讨三子养亲汤(SZ)对大鼠实验性支气管哮喘的保护作用及潜在机制。方法以卵清蛋白(OVA)致敏和激发诱发支气管哮喘,将大鼠随机分为5组:对照组、模型组、地塞米松(阳性药,1 mg/kg)组、SZ治疗(200 mg/kg)组和SZ单给药(不造模,200 mg/kg)组。试剂盒法检测血清免疫球蛋白E(IgE)水平。ELISA试剂盒法检测支气管肺泡灌洗液(BALF)中的炎性生物标志物白细胞介素(IL)-4、IL-5、IL-13、肿瘤坏死因子-α(TNF-α)、γ-干扰素(IFN-γ);Dunger’s稀释液染色法进行BALF中嗜酸性粒细胞计数;试剂盒法检测BALF中氧化氮应激生物标记物丙二醛(MDA)和谷胱甘肽(GSH)水平、超氧化物歧化酶(SOD)的活性;实时荧光定量PCR法检测BALF中诱导型一氧化氮合酶(iNOs)RNA水平。以Western blotting法检测肺匀浆中肺表面活性蛋白-D(SP-D)表达水平;免疫组化测定肺中尿皮质素(UCN)的表达。HE染色法观察大鼠肺组织病理学变化。结果与模型组比较,SZ治疗可显著降低血清IgE水平(P<0.05);显著降低BALF中MDA含量,同时显著提高SOD活性和GSH含量(P<0.05);显著降低BALF中IL-4、IL-5、IL-13、IFN-γ和TNF-α水平(P<0.05);显著降低肺组织中SP-D和UCN的蛋白表达(P<0.05);HE染色结果表明,SZ显著改善模型大鼠肺组织炎症病变。结论 SZ可通过下调SP-D和UCN表达改善OVA诱导的支气管哮喘。Objective Investigate the protective effect and mechanism of Sanziyangqin Decoction(SZ) on experimental bronchial asthma in rats. Methods Bronchial asthma was sensitized and stimulated with ovalbumin(OVA). Rats were randomly divided into five groups, including normal control group, model group, DEXA(positive drug, 1 mg/kg) group, SZ treatment(200 mg/kg) and SZ control(200 mg/kg) group. The level of serum immunoglobulin E(IgE) was detected by kit method. Interleukin(IL)-4, IL-5, IL-13,tumor necrosis factor-α(TNF-α) and interferon-γ(IFN-γ) in bronchoalveolar lavage fluid(BALF) were detected by ELISA kit;Dunger’s dilution staining method was used to count eosinophils in BALF;the levels of malondialdehyde(MDA), glutathione(GSH)and the activity of superoxide dismutase(SOD) in BALF were detected by kit method;the level of inducible nitric oxide synthase(iNOS) RNA in BALF was detected by real-time quantitative PCR. The expression of pulmonary surfactant protein-D(SP-D) in lung homogenate was detected by Western blotting, and the expression of urinary corticosteroids(UCN) was detected by immunohistochemistry. He staining was used to observe the pathological changes of lung tissue. Results Compared with the model group, SZ treatment significantly reduced the serum IgE level(P < 0.05), significantly reduced the MDA content in BALF,significantly increased the SOD activity and GSH content(P < 0.05), significantly reduced the levels of IL-4, IL-5, IL-13, IFN-γ and TNF-α in BALF(P < 0.05), significantly reduced the protein expression of SP-D and UCN in lung tissue(P < 0.05);HE staining showed that SZ significantly improved the model inflammatory changes of lung tissue in model rats. Conclusion SZ can improve the bronchial asthma induced by ovalbumin by regulating the expression of SP-D and UCN.

关 键 词：三子养亲汤 支气管哮喘 尿皮质素 肺表面活性蛋白-D 炎症 氧化应激 

分 类 号：R965[医药卫生—药理学]