基于UPLC-Q-TOF/HRMSE的活血解毒方化学成分定性分析研究  

Analysis of chemical constituents of Huoxue Jiedu Decoction extract by UPLC-Q-TOF/HRMS^E

作　　者：杨欣欣 刘传鑫 颜昌钖 常鑫鑫 李娅琦 王梓轩 肖治均 马长华[1] YANG Xinxin;LIU Chuanxin;YAN Changyang;CHANG Xinxin;LI Yaqi;WANG Zixuan;XIAO Zhijun;MA Changhua(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China;School of Pharmacy,Hebei College of Traditional Chinese Medicine,Shijiazhuang 050200,China)

机构地区：[1]北京中医药大学中药学院,北京102488 [2]河北中医学院药学院,河北石家庄050200

出　　处：《药物评价研究》2020年第11期2207-2216,共10页Drug Evaluation Research

基　　金：国家自然科学基金资助项目(81774014)。

摘　　要：目的采用超高效液相色谱-四级杆-飞行时间质谱(UPLC-Q-TOF/HRMSE)技术对活血解毒方中的化学成分进行研究。方法采用ACQUITY UPLC BEH C18色谱柱(100 mm×2.1 mm,1.8μm),以0.1%甲酸溶液(A)和0.1%甲酸乙腈(B)作为流动相进行梯度洗脱,体积流量为0.3 mL/min,采用ESI源在正、负离子模式下采集数据。根据化合物精确相对分子质量及二级特征碎片离子信息,结合参考文献数据与UNIFI数据库,鉴定活血解毒方的主要化学成分。结果从活血解毒方中鉴定出66个化学成分,其中黄酮类化合物8个、生物碱类化合物7个、酚类化合物6个、皂苷类化合物30个、木质素类化合物6个、萜类化合物4个和其他类化合物5个。结论建立的UPLC-Q-TOF/HRMSE联用技术与UNIFI结合法能够系统、快速、准确地鉴定多种化学成分,为其质量评价指标选择及药效物质基础深入研究提供了参考。Objective The chemical constituents of Huoxue Jiedu Decoction were studied by ultra-high performance liquid chromatography-four-stage rod-time-of-flight mass spectrometry(UPLC-Q-TOF/HRMSE). Method ACQUITY UPLC BEH C18 column(100 mm × 2.1 mm, 1.8 μm) was used for gradient elution with 0.1% formic acid aqueous solution(A) and 0.1% acetonitrile formate(B) as mobile phases. The volume flow rate was 0.3 mL/min. ESI source was used to collect data in positive and negative ion modes. According to the precise relative molecular weight and secondary fragment ion information of compounds, the main chemical constituents of Huoxue Jiedu Formula were identified with reference data and UNIFI database. Results Sixty-six chemical constituents were identified from Huoxue Jiedu Recipe, including eight flavonoids, seven alkaloids, six phenolics, 30 saponins, six lignins, four terpenoids and five other compounds. Conclusion The established UPLC-Q-TOF/HRMSE combined with UNIFI method can identify a variety of chemical components systematically, quickly and accurately, which provides a reference for the selection of quality evaluation indexes and in-depth study of the material basis of its efficacy.

关 键 词：活血解毒方 UPLC-Q-TOF/HRMSE UNIFI 化学成分 定性分析 黄酮类 生物碱类 酚类 皂苷类 木脂素类 萜类 

分 类 号：R284.1[医药卫生—中药学]