新型冠状病毒肺炎病例不同标本核酸检测结果分析  

Nucleic acid detection results analysis in different COVID-19 samples

作　　者：蒋立立[1] 潘献民 刘颖[1] 龙虎[1] 王宁[1] 李立巍 刘志冰[1] 黄杨 郭昕 王彦文[1] 李剑军[2] JIANG Lili;PAN Xianmin;LIU Ying;LONG Hu;WANG Ning;LI Liwei;LIU Zhibing;HUANG Yang;GUO Xin;WANG Yanwen;LI Jianjun(Guilin Center for Disease Control and Prevention,Guilin,Guangxi 541001,China;Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention,Nanning,Guangxi 530028,China)

机构地区：[1]桂林市疾病预防控制中心,广西桂林541001 [2]广西壮族自治区疾病预防控制中心,广西南宁530028

出　　处：《中国热带医学》2020年第12期1193-1196,共4页China Tropical Medicine

摘　　要：目的了解不同类型和疾病进展阶段新型冠状病毒肺炎(coronavirus disease 2019,COVID-19)病例的标本新型冠状病毒(SARS-CoV-2)核酸检测结果及影响因素。方法对桂林市新型冠状病毒肺炎疑似病例及密切接触者分别采集深咳痰液、咽拭子、粪便及眼结膜拭子,采用双重实时荧光RT-PCR方法进行SARS-CoV-2核酸检测,收集流行病学、临床实验室及辅助诊断等资料,分析提高核酸检测敏感性的因素。结果共采集537例COVID-19疑似病例及密切接触者标本654份,其中,深咳痰液569份,咽拭子26份,粪便28份,眼结膜拭子31份。SARS-CoV-2核酸阳性32例,核酸阳性检出率6.00%(32/537);首次核酸阳性的深咳痰液的采样时间与发病时间的间隔天数0~23 d。在2种样本检测比较中,深咳痰液中SARS-CoV-2核酸阳性检出率明显高于咽拭子标本的检出率(P=0.000)。粪便和眼结膜拭子中SARS-CoV-2核酸阳性分别有19例和5例,它们的采样时间与首次核酸阳性的深咳痰液样本的采样时间比较平均晚7 d。在32例新型冠状病毒肺炎确诊病例中,28例单次采集深咳痰液样本为核酸阳性,其中,于发病第1、2、3、4和5 d及以上分别有6、6、3、4和9例;4例病例需采集2次及以上深咳痰液样本才出现核酸阳性结果。1例病例在采样前有发热及治疗史,作为密切接触者排查隔离观察,分别于发病后第21天(阴性)、第23天(阳性)采集2份深咳痰液标本检测。结论SARS-CoV-2核酸检测中深咳痰液标本优于咽拭子标本,粪便和眼结膜拭子也可检测到病毒核酸,采样方式、采样时机及治疗史为核酸检测敏感性的影响因素,对疑似病例或密切接触者需隔离观察并多次采样检测,以免漏检。Objective To understand the results and influencing factors of SARS-CoV-2 nucleic acid detection among specimens in different types and stages of coronavirus disease 2019(COVID-19)progression.MethodsThe samples of deep-tussive sputum,pharyngeal swab,stool and conjunctival swab were collected among the suspected cases and close contacts of COVID-19 in Guilin.The SARS-CoV-2 RNAs were detected using double fluorescence real-time RT-PCR.The epidemiology,clinical diagnosis and laboratory data were collected to analyze the factors of improving the sensitivity of nucleic acid detection.ResultsA total of 654 samples were collected among 537 suspected cases and close contacts,including 569 deep-tussive sputum,26 pharyngeal swabs,28 stool and 31 conjunctival swabs.Thirty-two SARS-CoV-2 nucleic acid positive cases were found,with a nucleic acid positive rate of 6.00%(32/537).The median days(range)of the first nucleic acid positive deep-tussive sputum sampling time from onset day of illness were 0-23 days.The SARS-CoV-2 nucleic acid positive rate of deep-tussive sputum was significantly higher than that of pharyngeal swabs(P=0.000).The SARS-CoV-2 nucleic acid positive number of stool and conjunctival swabs were 19 cases and 5 cases,respectively,which their sampling time were the average 7 days later than that of the first sampling deep-tussive sputum with SARS-CoV-2 positive.Among the 32 confirmed cases of COVID-19,there were 28 cases collected a single sample of deep-tussive sputum with SARS-CoV-2 nucleic acid positive,which samples of 6 cases,6 cases,3 cases,4 cases and 9 cases were collected on the first day,2 nd day,3 rd day,4 th day,5 th day and above,respectively.In 4 cases,samples of deep-tussive sputum should be collected twice or more before nucleic acid positive results.Before sampling,one case had a history of fever and treatment.As a close contact,two samples of deep-tussive sputum were collected on the 21 st day and 23 rd day from onset day of illness,with SARS-CoV-2 nucleic acid negative and positive,respective

关 键 词：新型冠状病毒 痰液 咽拭子 粪便及眼结膜拭子 核酸检测 

分 类 号：R563.14[医药卫生—呼吸系统]