三七总皂苷改善慢性脑低灌注大鼠认知障碍的行为学研究  

Panax notoginseng saponin improves behaviors of cognitive impairment in chronic cerebral hypoperfusion rats

作　　者：李思宇 李余霞 王庆松 王丹 牟子超 王小艳 Li Siyu;Li Yuxia;Wang Qingsong;Wang Dan;Mu Zichao;Wang Xiaoyan(Department of Neurology,General Hospital of Western Theater,Chengdu 610083,Sichuan Province,China)

机构地区：[1]西部战区总医院神经内科,成都610083 [2]西南交通大学医学院

出　　处：《中华老年心脑血管病杂志》2020年第12期1316-1320,共5页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：四川省卫计委科研项目(16PJ014)。

摘　　要：目的探讨三七总皂苷(panax notoginseng saponins,PNS)对慢性脑低灌注(chronic cerebral hypoperfusion,CCH)大鼠认知障碍的改善作用。方法60只成年健康雄性SD大鼠按随机数字表法分为4组,假手术(Sham)组、CCH组、溶剂组(RJ组)、PNS组,每组15只。后3组采用永久性双侧颈总动脉阻断建立CCH模型,造模后,PNS组和RJ组每日分别给予5%PNS生理盐水溶液和生理盐水腹腔注射,连续4周。旷场实验观察大鼠站立次数、周边及中央区域活动情况,物体辨别实验观察物体辨别指数(DI),Morris水迷宫实验观察逃避潜伏期、游泳总路程、穿越次数和穿越潜伏期。结果与Sham组比较,CCH组站立次数、中央区域活动时间、DI、总探索时间、穿越次数明显减少,周边区域活动时间、游泳总路程、避潜伏期和穿越潜伏期明显增加,差异有统计学意义(P<0.05)。与RJ组比较,PNS组站立次数明显增加[(22.33±1.50)次vs(12.92±1.98)次,P<0.05],周边区域活动时间明显减少[(272.1±27.7)s vs(291.9±16.2)s,P<0.05],中央区域活动时间明显增加[(28.5±27.7)s vs(9.5±16.5)s,P<0.05];DI明显增加(0.63±0.36 vs 0.27±0.26,P<0.05),总探索时间明显增加[(77.42±71.83)s vs(26.84±38.10)s,P<0.05];游泳总路程和避潜伏期显著减少(P<0.05);穿越次数明显增加[(2.50±1.01)次vs(1.08±0.99)次,P<0.05],穿越潜伏期明显减少,差异有统计学意义[(11.48±3.62)s vs(34.07±9.97)s,P<0.05]。结论PNS可明显改善CCH大鼠的空间学习和记忆能力、情绪唤醒水平、自发性活动水平、探索能力、识别记忆能力等。Objective To study the effect of Panax notoginseng saponins(PNS)on cognitive impairment in chronic cerebral hypoperfusion(CCH)rats.Methods Sixty adult healthy male SD rats were randomly divided into sham operation group,CCH group,RJ group and PNS group(15 in each group).After a CCH model of rats was established by permanent occlusion of bilateral common carotid arteries,the rats in PNS group and RJ group received intraperitoneal injection with 5%PNS physiological saline solution and normal saline(once a day)for 4 weeks.Following data were recorded,including standing times,activity time in surrounding and central areas,object discrimination index(DI),and escape latency,total swimming distance,crossing times,crossing latency in Morris water maze test.Results The standing times and crossing times were significantly less,the object DI was significantly lower,the activity time in central area and total exploration time were significantly shorter while the activity time in surrounding area,total swimming distance,escape latency and crossing latency were significantly longer in CCH group than in sham operation group(P<0.05).The standing times were significantly more,the activity time in surrounding area was significantly shorter,the activity time in central area was significantly lon-ger,the object DI was significantly higher,the total exploration time was significantly longer,the total swimming distance and escape latency were significantly shorter,the crossing time was significantly longer and the crossing latency was significantly shorter in PNS group than in RJ group(22.33±1.50 vs 12.92±1.98,P<0.05;272.1±27.7 s vs 291.9±16.2 s,P<0.05;28.5±27.7 s vs 9.5±16.5 s,P<0.05;0.63±0.36 vs 0.27±0.26,P<0.05;77.42±71.83 s vs 26.84±38.10 s,P<0.05;2.50±1.01 vs 1.08±0.99,P<0.05;11.48±3.62 s vs 34.07±9.97 s,P<0.05).Conclusion PNS can effectively improve the spatial learning and memory ability,emotional arousal level,spontaneous activity level,exploration ability,recognition and memory ability of CCH rats.

关 键 词：三七皂甙 脑血管循环 认知障碍 空间学习 记忆 

分 类 号：R285.5[医药卫生—中药学]