耐热植酸酶的实验室评估方法  

Laboratory evaluation of thermostable phytase

作　　者：刘胜利 刘示杰 刘文龙 马传兴 王克芬 陆利霞[3] LIU Shengli;LIU Shijie;LIU Wenlong;MA Chuanxing;WANG Kefen;LU Lixia(Shandong Lonct Enzymes Co.,Ltd.,Linyi 276400,China;College of Polymer Science and Engineering,Qingdao University of Science and Technology,Qingdao 266042,China;College of Food Science and Light Industry,Nanjing Tech University,Nanjing 211800,China)

机构地区：[1]山东隆科特酶制剂有限公司,山东临沂276400 [2]青岛科技大学高分子科学与工程学院,山东青岛266042 [3]南京工业大学食品与轻工学院,江苏南京211800

出　　处：《生物加工过程》2020年第6期766-774,共9页Chinese Journal of Bioprocess Engineering

摘　　要：本研究旨在研究实验室评估耐热植酸酶的方法。以市场销售量比较大的7种植酸酶为研究对象,分别模拟其在胃肠消化液中的酶活留存率,水浴法模拟高温制粒过程中的酶活留存率等指标来评判耐热植酸酶的优劣;同时在仿生条件下分析耐热植酸酶酶解豆粕的效果。各耐热植酸酶用水浴法(3 min 90℃)后测得留存酶活和生产实际85℃调制制粒的留存酶活基本一致,差异不显著(P>0.05),故用水浴法(3 min 90℃)评估生产实际调制制粒后的耐热植酸酶的酶活留存率是可行的。依据模拟消化系统实验室评价为饲用耐热植酸酶的有效性提供方法及参考指标,并为生产实践中耐热植酸酶的使用提供数据支撑。The purpose of this study is to evaluate a laboratory assay for heat-resistant phytase.Seven large-sales phytase were bought as test samples.The indexes such as enzyme survival rate in simulated gastrointestinal fluid and in simulated high-temperature granulation process in water bath were tested to judge the activity of heat-resistant phytase.Moreover,the indexes of enzymatic hydrolysis of soybean meal by the heat-resistant phytases were analyzed.The activity of different heat-resistant phytase in water bath at 90℃for 3 minutes could be used to evaluate that at 85℃in practice production.There was no significant difference(P>0.05).The methods and their indexes were adapted to evaluate the effectiveness of feeding heat-resistant phytase based on simulated digestive system in vitro.And the results were provided for the use of heat-resistant phytase in feed production practice.

关 键 词：耐热植酸酶 酶活留存率 植酸磷释放量 饲料添加剂 

分 类 号：S816[农业科学—饲料科学]