黄精挥发油提取工艺的响应面法优化及抗氧化活性研究  

Optimization of extraction process by Response surface methodology and antioxidant activity of volatile oil from Polygonatum Rhizoma

作　　者：逯莉[1] 刘峰 介磊 豆佳媛 彭修娟[1] LU Li;LIU Feng;JIE Lei;DOU Jiayuan;PENG Xiujuan(Collaborative Innoration Center of Green Manufacturing Technology of Traditional Chinese Medicine in Shaanxi Province,Shaanxi International Business College,Xianyang,Shaanxi,712046 P.R.China;Shaanxi Buchang Pharmaceutical Limited Company,Xi'an,Shaanxi,710075 P.R.China;Shaanxi Kunghui Pharmaceutical Co.,Ltd.,Xianyang,Shaansi,712000 P.R.China)

机构地区：[1]陕西国际商贸学院陕西省中药绿色制造技术协同创新中心,陕西咸阳712046 [2]陕西步长制药有限公司,陕西西安710075 [3]陕西康惠制药股份有限公司,陕西咸阳712000

出　　处：《华西药学杂志》2020年第6期613-616,共4页West China Journal of Pharmaceutical Sciences

基　　金：陕西省科技厅项目(2012K19-04-06);陕西省生物医药创新制药技术研究院士专家工作站(陕科协发[2017]事企字15号);陕西省中医药管理局项目(ZYPT001)。

摘　　要：目的通过响应面法优化超声波辅助提取黄精挥发油的工艺,并研究黄精挥发油的体外抗氧化活性。方法以提取溶剂、液料比、提取时间、提取温度为考察因素,在单因素实验的基础上,利用响应面法优化黄精挥发油的提取工艺。通过还原Fe3+能力和清除DPPH自由基的能力考察黄精挥发油的抗氧化活性。结果最佳提取溶剂为石油醚,提取工艺液料比为10:1,提取时间20 min,提取温度31℃,黄精中挥发油的得率2.035%。黄精挥发油还原Fe3+能力、清除DPPH自由基的能力与浓度呈正相关。结论优化的黄精挥发油提取工艺稳定,提取率高;黄精挥发油具有较强的抗氧化活性,可为进一步开发利用黄精药材提供依据。OBJECTIVE To optimize the process of ultrasonic-assisted extraction of volatile oil from Polygonatum sibiricum Red.by Response surface methodology and to study the antioxidant activity of the volatile oil of P.sibiricum.METHODS The extraction solvent,liquid to material ratio,extraction time and extraction temperature were taken as the investigation factors.On the basis of single factor experiment,the response surface method was used to optimize the extraction process of volatile oil from P.sibiricum.The antioxidant activity of the volatile oil of P.sibiricum was investigated by reducing the ability of Fe3+and scavenging DPPH free radicals.RESULTS By using the best extraction solvent petroleum ether,the best extraction process liquid to material ratio of 10:1,extraction time 20 min,and extraction temperature 31℃,the yield of volatile oil in PR was 2.035%.The ability of the volatile oil of P.sibiricum to reduce Fe3+and the ability to scavenge DPPH free radicals were positively correlated with the concentration.CONCLUSION The optimized extraction process of volatile oil from P.sibiricum is stable and the extraction rate is high.The volatile oil of PR has strong antioxidant activity,which provides a scientific basis for further development and utilization of P.sibiricum.

关 键 词：黄精 挥发油 单因素 提取工艺 超声 响应面法 优化 抗氧化活性 

分 类 号：R917[医药卫生—药物分析学] R96[医药卫生—药学]