大花红景天的HPLC指纹图谱研究  

Study on HPLC fingerprint of Rhodiola crenulata

作　　者：黄小清 土旦卓玛[2] 殷钲皓 余倩倩 韩泳平 HUANG Xiaoqing;TU Danzhouma;YIN Zhenghao;YU Qianqian;HAN Yongping(College of Pharmacy,Southwest Minzu University Chengdu,Sichuan,610041 P.R.China;School of Medicine,Tibet University,Lhasa,Tibet,85000 P.R.China)

机构地区：[1]西南民族大学药学院,四川成都610041 [2]西藏大学医学院,西藏拉萨850000

出　　处：《华西药学杂志》2020年第6期635-639,共5页West China Journal of Pharmaceutical Sciences

基　　金：国家十二五科技支撑计划(2012BAI27B07);西南民族大学2019年研究生创新型科研项目(CX2019SZ171)。

摘　　要：目的采用HPLC指纹图谱同时测定大花红景天中的红景天苷、酪醇和洛塞维。方法采用Cosmosil 5 C18-MS-Ⅱ色谱柱(250 mm×4.6 mm,5μm),流动相为0.1%甲酸溶液-乙腈,梯度洗脱,流速1.0 mL·min^-1,检测波长277 nm,建立了10批样品的指纹图谱;并测定样品中红景天苷、酪醇和洛塞维的含量。结果在10批样品的指纹图谱中确定了16个共有峰,样品相似度为0.942~0.991;与对照品比较,指认出红景天苷、酪醇和洛塞维成分;不同产地的10批大花红景天样品可分为3类;样品中红景天苷、酪醇和洛塞维的含量分别为0.69%~1.76%、0.16%~0.45%、0.07%~0.90%。结论所用方法快速实用、重复性好,在药材的质量控制方面有重要的价值。OBJECTIVE To simultaneously determine indicator components of salidroside,tyrosol and rosavin in Rhodiola crenulata by HPLC fingerprint.METHODS Cosmosil 5 C18-MS-Ⅱ column(250 mm×4.6 mm,5μm)was used as chromatographic column.0.1% aqueous formic acid and acetonitrile were used as mobile phase with gradient elution at a flow rate of 1.0 mL·min^-1.Detection wavelength was 277 nm.The fingerprints of 10 batches of samples were established.Contents of salidroside,tyrosol and rosavin in the samples were determined.RESULTS Sixteen common peaks were confirmed in the fingerprints of 10 batches of samples,and sample similarities were 0.942-0.991.After comparison with the reference substance,three components of salidroside,tyrosol and rosavin were identified.10 batches of R.crenulata samples from different regions were divided into 3 categories.The contents of salidroside,tyrosol and rosavin in the samples were 0.69%-1.76%,0.16%-0.45% and 0.07%-0.90%,respectively.CONCLUSION The method is fast and practical,has good repeatability,and has important application value in the quality control of medicinal materials.

关 键 词：大花红景天 高效液相色谱法 指纹图谱 红景天苷 酪醇 洛塞维 含量测定 质量控制 模式识别 

分 类 号：R917[医药卫生—药物分析学]