Combined application of extended depth of field imaging, image stitching and polarized microscopy techniques in identification of Spatholobus suberectus  

作　　者：Ying-xian Ma Chayanis Sutcharitchan Xiao-die Li Qian Meng Xin Wang Shen Ji Ya-jun Cui 

机构地区：[1]Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China [2]Chulalongkorn University,Bangkok 10330,Thailand [3]Shanghai Institute for Food and Drug Control,Shanghai 201203,China

出　　处：《Chinese Herbal Medicines》2020年第4期367-374,共8页中草药（英文版）

基　　金：financially supported by National Key R&D Program of China(No.2018YFC1707900)。

摘　　要：Objective:As traditional techniques for microscopic identification of Chinese medicines currently lack objective and high-quality reference images,here we developed a systemic procedure to be used in microscopic identification of Chinese medicines,which would lead to more objective,effective and accurate identification process.Methods:Spatholobi Caulis(Jixueteng in Chinese)was used as the specimen in the development of such procedure.Jixueteng samples were microscopically examined in bright-and dark-field microscopy.Microscopic images were obtained by regular,EDF,and image stitching techniques.Results:The microscopic images of the characteristics in pulverized Jixueteng were captured,thanks to EDF imaging and image stitching techniques which allowed the detailed and full sighting of each characteristic to be obtained simultaneously.Different layers in anatomical transverse section,including cork,phelloderm,cortex,phloem,cambium,xylem and pith,were distinctively observed.Moreover,by comparing images of bright-and dark-field microscopy,birefringent and non-birefringent components could readily be distinguished.Conclusion:With application of the developed procedure,high-definition,panoramic and microscopic images were acquired,which could be used as the reference images for microscopic identification of Chinese medicines.

关 键 词：extended depth of field imaging image stitching microscopic identification polarized light PHARMACOGNOSY spatholobus suberectus Dunn 

分 类 号：R282.5[医药卫生—中药学]