Enzymatic synthesis of myricetin 3-O-galactoside through a whole-cell biocatalyst  

作　　者：Zhen Xu Wei-qing He Chun-sheng Liu Jian-qiang Kong 

机构地区：[1]State Key Laboratory of Bioactive Substance and Function of Natural Medicines&NHC Key Laboratory of Biosynthesis of Natural Products,Institute of Materia Medica,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100050,China [2]School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China [3]NHC Key Laboratory of Biotechnology of Antibiotics,Institute of Medicinal Biotechnology,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100050,China

出　　处：《Chinese Herbal Medicines》2020年第4期384-389,共6页中草药（英文版）

基　　金：supported by National Mega-project for Innovative Drugs(2018ZX09711001-006);CAMS Innovation Fund for Medical Sciences(CIFMS)(2016-I2M-3-012 and 2019-I2M-1005);Disciplines Construction Project(201920100801);Beijing Natural Science Foundation(7172143)。

摘　　要：Objective:Myricetin 3-O-galactoside is an active compound with pharmaceutical potential.The insufficient supply of this compound becomes a bottleneck in the druggability study of myricetin 3-Ogalactoside.Thus,it is necessary to develop a biosynthetic process for myricetin 3-O-galactoside through metabolic engineering.Methods:Two genes OcSUS1 and OcUGE1 encoding sucrose synthase and UDP-glucose 4-epimerase were introduced into BL21(DE3)to reconstruct a UDP-D-galactose(UDP-Gal)biosynthetic pathway in Escherichia coli.The resultant chassis strain was able to produce UDP-Gal.Subsequently,a flavonol 3-O-galactosyltransferase DkFGT gene was transformed into the chassis strain producing UDP-Gal.An artificial pathway for myricetin 3-O-galactoside biosynthesis was thus constructed in E.coli.Results:The obtained engineered strain was demonstrated to be capable of producing myricetin 3-Ogalactoside,reaching 29.7 mg/L.Conclusion:Biosynthesis of myricetin 3-O-galactoside through engineered E.coli could be achieved.This result lays the foundation for the large-scale preparation of myricetin 3-O-galactoside.

关 键 词：engineered Escherichia coli flavonol 3-O-galactosyltransferase GALACTOSYLTRANSFERASE myricetin 3-O-galactoside 

分 类 号：R284.3[医药卫生—中药学]