表面活性剂双水相萃取牛血清白蛋白的影响因素  

Influencing factors of aqueous surfactant two phases extraction of bovine serum albumin

作　　者：王新刚 杨效益[1] 孙永强[1] 郭朝华[1] 李萍[1] 李建波[1] WANG Xin′gang;YANG Xiaoyi;SUN Yongqiang;GUO Chaohua;LI Ping;LI Jianbo(China Research Institute of Daily Chemical Industry Co.Ltd.,Taiyuan 030001,China)

机构地区：[1]中国日用化学工业研究院有限公司,山西太原030001

出　　处：《印染助剂》2020年第12期36-39,共4页Textile Auxiliaries

基　　金：“十三五”国家科技支撑计划(2017YFB0308802);伽蓝研究基金(JALA2016)。

摘　　要：阴离子表面活性剂窄分布脂肪醇醚硫酸钠(N-AE5S)与阳离子表面活性剂十四烷基三甲基溴化铵(TTAB)复配过程中可以形成双水相体系(ASTP),且可将牛血清白蛋白(BSA)成功地从水溶液中萃取出来。探究了表面活性剂浓度、N-AE5S/TTAB物质的量比对双水相形成区域及萃取率的影响。结果表明:在表面活性剂浓度为6.0×10^-2mol/L、N-AE5S/TTAB物质的量比为4.2∶5.8时,ASTP对BSA的萃取率达66.05%。Anionic surfactant fatty alcohol polyethyleneglycol ether sulfates with narrow ethylene oxide distribution(N-AE5S)and cationic surfactant tetradecyltrimethylammonium bromide(TTAB)could form aqueous surfactant two phases(ASTP)during the compounding process.And ASTP could successfully extract bovine serum albumin(BSA)from aqueous solution.The effects of surfactant concentration and molar ratio of NAE5S to TTAB on formation area of ASTP and extraction rate were investigated.The results showed that when the surfactant concentration was 6.0×10^-2 mol/L and the molar ratio of N-AE5S to TTAB was 4.2∶5.8,the extraction rate of ASTP to BSA was 66.05%.

关 键 词：脂肪醇醚硫酸钠 窄分布 牛血清白蛋白 萃取 表面活性剂双水相 

分 类 号：TQ423[化学工程]