山麻黄不同炮制品中麻黄碱与伪麻黄碱含量测定  

Determination of ephedrine and pseudoephedrine in different processed products of EPhedra likiangensi Florin

作　　者：吴珊珊 覃信 陈学艳 熊瑞 李玮 何席呈 WU Shan-shan;QIN Xing;CHEN Xue-yan;XIONG Rui;LI Wei;HE Xi-cheng(Guizhou Universily of Traditional Chinese Medicine,College of Pharmacy,Guiyang,550025 China;Guizhou Provincial Key Laboratory of Miao Medicine,Guiyang,550025 China)

机构地区：[1]贵州中医药大学药学院,贵州贵阳550025 [2]贵州省苗医药重点实验室,贵州贵阳550025

出　　处：《时珍国医国药》2020年第8期1881-1883,共3页Lishizhen Medicine and Materia Medica Research

基　　金：贵州省教育厅青年科技人才成长项目(黔教合KY字[2018]208);贵州省苗医药重点实验室开放课题(黔苗医药K字[2017]038);贵州省一流学科建设项目(GNYL[2017]008号-7);贵州中医药大学博士启动基金(2017)。

摘　　要：目的探讨不同炮制方法对山麻黄中麻黄碱,伪麻黄碱的影响。方法分析采用Waters Bridge C18柱(4.6 mm×150mm,5μm);流动相为乙腈-20 mmoL/L磷酸氢二氨水溶液(用氨水调pH=10)溶液,梯度洗脱;流速为1.0 mL/min;柱温为30℃;检测波长为215 nm。结果麻黄碱、伪麻黄碱分别在0.1454~2.9080 μg(r=0.9999),0.0848~1.6960 μg(r=0.9998)范围内线性关系良好,盐酸麻黄碱的加样回收率在96.27%-99.88%之间,盐酸伪麻黄碱的加样回收率在95.39%~98.66%之间,RSD值分别为1.54%和1.12%。结论该方法准确稳定,重复性好,可为山麻黄饮片的质量控制提供参考,也为其临床应用提供依据。Objective To study the effects of ephedrine and pseudoephedrine in different processed products of EPhedra likiangensi Florin.Methods The analysis of EPhedra likiangensi Florin was performed on a Waters Bridge column(4.6 mm×150 mm,5 μm), with the mobile phase comprising of acetonitrile-20 mmoL/L diammonium phosphate flowing at 1. 0 mL/min in a gradient elution manner, and the detection wavelength was set at 215 nm. Results Ephedrine and pseudoephedrine showed good linear relationships within the ranges of 0.1454~2.9080 μg(r=0.9999) and 0.0848~1.6960 μg(r=0.9998), whose ranges of recoveries were 96.27%~99.88% and 95.39%~98.66%, RSD were 1.54% and 1.12%.Conclusion The method is accurate, stable and repeatable, which can provide reference for quality control of EPhedra likiangensi Florin and basis for its clinical application.

关 键 词：山麻黄 炮制 麻黄碱 伪麻黄碱 

分 类 号：R283.1[医药卫生—中药学]