迟缓爱德华菌麦芽糖孔道蛋白LamB的免疫特性  

Prokaryotic expression and immunological protection analysis of LamB protein of Edwardsiella tarda

作　　者：杜万年 王苗 葛成 刘晨 于秀剑 郑欢亭 李晓丹 刘勃兴 吴同垒[1] 史秋梅[1] 张志强 DU Wan-nian;WANG Miao;GE Cheng;LIU Chen;YU Xiu-jian;ZHENG Huan-ting;LI Xiao-dan;LIU Bo-xing;WU Tong-lei;SHI Qiu-mei;ZHANG Zhi-qiang(College of Animal Science and Technology,Hebei Normal University of Science&Technology,Qinhuangdao,Hebei 066000,China)

机构地区：[1]河北科技师范学院动物科技学院,河北秦皇岛066004

出　　处：《中国兽医学报》2020年第11期2176-2180,共5页Chinese Journal of Veterinary Science

基　　金：“农业部淡水渔业健康养殖重点实验室、浙江省鱼类健康与营养重点实验室/浙江省淡水水产遗传育种重点实验室”开放基金课题资助项目(ZJK201812);中国博士后科学基金资助项目(2017M611935);河北科技师范学院海洋专项资助项目(2018HY007)。

摘　　要：LamB是革兰阴性细菌编码的麦芽糖孔道蛋白,介导细菌对胞外营养物质的摄取,在弧菌的研究中证实该蛋白作为免疫原使用具有优良的保护效果。但迟缓爱德华菌LamB蛋白的疫苗潜力研究尚未见报道,本研究利用大肠杆菌表达系统对迟缓爱德华菌LamB进行原核表达和纯化,进一步将重组蛋白rLamB免疫小鼠和斑马鱼,评测其免疫原性和保护效果。结果显示,成功表达和纯化出重组LamB蛋白,相对分子质量约为45000。小鼠模型显示,rLamB免疫小鼠能够激发高水平的抗体。斑马鱼感染试验表明,rLamB免疫能够一定程度保护斑马鱼免受迟缓爱德华菌ET-CL强毒株感染,保护率为65%。本研究证实了迟缓爱德华菌rLamB蛋白的免疫原性和潜在的疫苗价值,为迟缓爱德华菌疫苗研制提供了参考和借鉴。LamB,a matloporin of gram-negative bacteria,is agent for the transport of maltose and maltodextrin to control the concentration of maltose in the cell bacterial stress and drug resistance.In a present study,LamB was reported to be is a versatile vaccine candidate among the Vibrio species.However,this protein in Edwardsiell tarda is not elucidate well,and in this study,we performed this protein for prokaryotic expression,purification,and further examined the immunogenicity and immunoprotection through mice and zebria fish models.The data showed that,the recombinant protein rLamB with the weight of 45000 is well expressed and purified.The rLamB was able to stimulate strong humoral immune response with high level of specific antibody.The data from zebra fish infection model showed that,the rLamB protein was able to protect zebra fish against E.tarda infection with protection rate up to 65%.All these work provides guidance and foundation for development of subunit vaccine of E.tarda.

关 键 词：迟缓爱德华菌 麦芽糖孔道蛋白 LAMB 免疫保护力 

分 类 号：S852.61[农业科学—基础兽医学]