红花CtMYB44基因核心片段的克隆与序列分析  

Cloning and sequence analysis of CtMYB44 gene fragment from Carthamus tinctorius

作　　者：连瑞丽[1] 李宇伟[1] 卢婷婷[1] 赵丽[1] 李利红[1] 姬向波[1,2] 唐桂芬 伊立阳 孙宇峥 王霞 LIAN Ruili;LI Yuwei;LU Tingting;ZHAO Li;LI Lihong;JI Xiangbo;TANG Guifen;YI Liyang;SUN Yuzheng;WANG Xia(College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou Henan 450046;Experimental and Research Center of Henan University of Animal Husbandry and Economy, Zhengzhou Henan 450046;Henan Key Laboratory of Innovation and Utilization of Unconventional Feed Resources, Zhengzhou Henan 450046;Century Park of Zhengzhou City, Zhengzhou Henan 450000)

机构地区：[1]河南牧业经济学院动物医药学院,河南郑州450046 [2]河南牧业经济学院实验研究中心,河南郑州450046 [3]河南省非常规饲料资源创新利用重点实验室,河南郑州450046 [4]郑州市世纪公园,河南郑州450000

出　　处：《现代牧业》2020年第4期14-18,共5页Modern Animal Husbandry

基　　金：河南牧业经济学院“中兽药生药学”重点培育学科(41000003)。

摘　　要：红花主要有效成分是黄酮类物质,MYB转录因子在黄酮类化合物合成的过程中起着非常重要的作用。本研究以红花花瓣作为实验材料,通过RT-PCR方法扩增出红花MYB44基因的核心片段,测序结果显示其长度为723 bp,命名为CtMYB44。同源性分析表明:红花CtMYB44基因片段与猴面花、野草莓、野大豆、苹果、白梨、三裂叶薯、醉蝶花同源性较高,具有高度的保守性。Flavonoids were the main effective components of Carthamus tinctorius.MYB transcription factors played an important role in the synthesis of flavonoids.In this study,one core fragment of MYB gene named CtMYB44 was cloned from the flower of Carthamus tinctorius by RT-PCR.The sequencing results showed that the fragment was 723 bp.The homology analysis showed that CtMYB44 gene fragment in Carthamus tinctorius had high homology and conservation with Erythranthe guttatus、Fragaria vesca、Glycine soja、Malus domestica、Pyrus bretschneideri、Ipomoea triloba and Tarenaya hassleriana.

关 键 词：红花 CtMYB44基因 克隆 序列分析 

分 类 号：S854[农业科学—临床兽医学]