红松籽油中皮诺敛酸富集纯化工艺  

Enrichment and Purification Technology of Pinolenic Acid from Pinus koraiensis Seed Oil

作　　者：包三三 王希清[1] 杨雨春[2] 武晓丹[1] 赵春建[1] 付玉杰[1,3] BAO Sansan;WANG Xiqing;YANG Yuchun;WU Xiaodan;ZHAO Chunjian;FU Yujie(College of Chemistry,Chemical Engineering and Resource Utilization,Northeast Forestry University,Harbin 150040,China;Jilin Provincial Academy of Forestry Sciences,Changchun 130031,China;College of Forestry,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]东北林业大学化学化工与资源利用学院,黑龙江哈尔滨150040 [2]吉林省林业科学研究院,吉林长春130031 [3]北京林业大学林学院,北京100083

出　　处：《林产化学与工业》2020年第6期23-29,共7页Chemistry and Industry of Forest Products

基　　金：国家重点研发计划资助项目(2016YFD0600805);黑龙江省科技攻关计划资助项目(GX18B003);高等学校学科创新引智计划(B20088)。

摘　　要：采用油脂乙醇醇解法结合尿素包埋法富集纯化红松籽油中的皮诺敛酸,在考察尿素与红松籽油脂肪酸乙酯质量比、乙醇与尿素比例、包合温度及包合时间等因素对皮诺敛酸乙酯GC含量影响的基础上,通过响应面法优化了尿素包合富集纯化的工艺条件。研究结果表明:皮诺敛酸乙酯的最佳纯化条件为尿素与红松籽油脂肪酸乙酯质量比3∶1、乙醇与尿素比例6∶1(mL∶g)、包合温度-13℃和包合时间24 h,在此条件下单次尿素包合后,皮诺敛酸乙酯纯度达94.75%,回收率达66.25%,纯度相比传统方法提高107%,回收率提高143%,且尿素在回收实验中表现出较好的重复性,3次包合和回收后皮诺敛酸的平均纯度仍达85.28%。The alcoholysis of oils and fats combined with urea inclusion method was used to enrich and purify pinolenic acid from the Pinus koraiensis seed oil for the first time.On the basis of investigating the influence of the ratio of P.koraiensis seed oil fatty acid ethyl esters to urea,ratio of ethanol to urea,inclusion temperature and inclusion time on the GC content of pinolenic acid ethyl ester,the enrichment and purification conditions of urea inclusion were optimized by response surface methodology.The results showed that the best purification conditions of pinolenic acid ethyl ester were the ratio of urea to P.koraiensis seed oil fatty acid ethyl esters 3∶1,the ratio of ethanol to urea 6∶1(mL∶g),the inclusion temperature-13℃and the inclusion time 24 h.Under the above optimal conditions,the purity of pinolenic acid ethyl ester could reach 94.75%,and the recovery rate could reach 66.25%.Compared with the traditional method,the purity increased by 107%,and the recovery rate increased by 143%.In addition,urea showed excellent reusability in the recovery experiment.The average purity of pinolenic acid could still reach 85.28%after three cycles of urea inclusion and recovery.

关 键 词：红松籽油 皮诺敛酸 富集纯化 尿素包合法 油脂醇解法 

分 类 号：TQ35[化学工程]