藻酸盐凝胶对糖尿病大鼠创面组织中组织蛋白酶S表达影响的研究  

The effects of alginate gel on expression of cathepsin S from the wound tissue in diabetes rats

作　　者：唐颖 赵晓彤[1] 陈明卫[1] 荚泽国 李雪婷 罗莉[1] 章诗琪[1] 方朝晖 TANG Ying;ZHAO Xiaotong;CHEN Mingwei(Department of Endocrinology,The First Affiliated Hospital of Anhui Medical University,Hefei 230032,China)

机构地区：[1]安徽医科大学第一附属医院内分泌科,合肥230032 [2]安徽省中医药科学院中医药糖尿病防治研究所

出　　处：《中国糖尿病杂志》2020年第11期861-865,共5页Chinese Journal of Diabetes

基　　金：安徽省重点研究与开发计划项目(202004a07020016)。

摘　　要：目的探讨藻酸盐凝胶对DM大鼠创面组织中组织蛋白酶S(Cat S)表达影响及Cat S在藻酸盐凝胶促进DM大鼠创面愈合过程中的作用。方法建立DM大鼠皮肤缺损模型,将大鼠分为正常对照(Con)组、模型组(Mod)和藻酸盐组(Alg),每组各12只。Con组伤口涂抹生理盐水,Mod组伤口涂抹生理盐水,Alg组伤口涂抹藻酸盐凝胶。观察3组创面愈合情况,RT⁃PCR和和Western blot检测创面愈合过程中Cat S mRNA及Cat S蛋白表达水平。结果与Con组比较,Mod、Alg组2、4、6 d时创面面积较大,创面愈合率较低(P<0.05或P<0.01)。与Mod组比较,Alg组创面面积较小,创面愈合率较高(P<0.05或P<0.01)。与同组0 d比较,各组6 d时创面面积均减少,创面愈合率均增加(P<0.01)。Mod、Alg组0、2、4、6 d时创面组织中Cat S蛋白和mRNA表达水平高于Con组(P<0.05或P<0.01)。Alg组2、4、6 d时Cat S蛋白和mRNA表达水平低于Mod组(P<0.05),与0 d时比较,各组6 d时Cat S蛋白和mRNA表达均降低(P<0.05或P<0.01)。结论DM大鼠创面组织中Cat S表达升高。藻酸盐凝胶可降低DM大鼠创面组织中Cat S表达,促进创面愈合。Objective To explore the effects of alginate gel on expression of cathepsin S(Cat S)from the wound tissue in diabetic rats and the role of alginate gel in the healing process of diabetic wounds.Methods Model of skin defect in diabetic rats was established.Diabetic rats were divided into normal control(Con)group,model group(Mod)and alginate group(Alg),with 12 rats in each group.Con group was smeared with saline,Mod group was smeared with saline,and Alg group was smeared with alginate gel.The wound healing of the three groups was observed.RT⁃PCR used to detect Cat S mRNA and Western blot was used to detect the expression of protein during wound healing.Results Compared with Con group,Mod and Alg groups had larger wound area and lower wound healing rate at 2,4 and 6 d(P<0.05 or P<0.01).Conversely,compared with Mod group,Alg group had smaller wound area and higher wound healing rate(P<0.05 or P<0.01).In addition,compared with 0 d in the same group,the wound area decreased and the wound healing rate increased on the 6th day(P<0.01).At 0,2,4,6 d,the expression levels of Cat S protein and mRNA in Mod and Alg groups were higher than those in Con group(P<0.05 or P<0.01).The expression levels of Cat S protein and mRNA in Alg group were lower than those in Mod group at 2,4,6 d(P<0.05).Compared with 0 d,the expression of Cat S protein and mRNA in Alg group was lower than that in Mod group at 6 d(P<0.05 or P<0.01).Conclusion The significantly up⁃regulated expression of Cat S was observed in the diabetic rat skin wound.Alginate gel could promote diabetic wound healing by decreasing Cat S expression from the wound tissue.

关 键 词：藻酸盐凝胶 糖尿病创面 大鼠 组织蛋白酶S 

分 类 号：R641[医药卫生—外科学]