短期严重大气及细颗粒物污染对大鼠眼后段炎性因子表达的影响  

Effect of short-term and severe fine particulate matter and air pollution on inflammatory cytokine in posterior segment of eyes in rats

作　　者：石圆圆 王欣 罗灵 许倩倩 司少艳 聂闯 石琳 秦亚亚 宫玉波 SHI Yuanyuan;WANG Xin;LUO Ling;XU Qianqian;SI Shaoyan;NIE Chuang;SHI Lin;QIN Yaya;GONG Yubo(Department of Ophthalmology the PLA Stragetic Support Force Characteristic Medical Center,Beijing 100101,China;Laboratory of Environmental Sense Organ Injury And Stress the PLA Stragetic Support Force Characteristic Medical Center,Beijing 100101,China;Center for Special Medicine And Experimental Research the PLA Stragetic Support Force Characteristic Medical Center,Beijing 100101,China)

机构地区：[1]战略支援部队特色医学中心眼科,北京100101 [2]战略支援部队特色医学中心国家环境保护环境感官应激与健康重点实验室,北京100101 [3]战略支援部队特色医学中心,特种医学实验研究中心,北京100101

出　　处：《解放军医学院学报》2020年第10期1001-1005,1010,共6页Academic Journal of Chinese PLA Medical School

基　　金：国家环境保护环境感官应激与健康重点实验室开放基金(19ZX84)。

摘　　要：目的研究短期严重大气污染暴露下大鼠眼球后段炎性因子表达的变化。方法将36只健康SPF级雌性SD大鼠随机分为正常对照组、大气污染组和细颗粒大气污染组,每组12只。正常对照组大鼠在原SPF级环境中常规饲养,不做任何暴露处理;大气污染组大鼠在暴露舱中暴露于外界重度污染空气;细颗粒大气污染组大鼠在暴露舱中暴露于滤除了大于2.5μm颗粒的污染空气中。两个暴露组的暴露时间均为6 d。在结束暴露后同时取3组大鼠眼球。采用多因子芯片检测大鼠眼后段IL-1β、IL-5、IL-18、MCP-1的表达。经苏木精-伊红染色后在光镜下观察大鼠眼后段切片的一般形态。酶联免疫吸附法检测大鼠血清IL-1β浓度。结果对照组和两个暴露组眼后段组织IL-1β浓度中位数分别为1.72(1.56,2.24)pg/ml、2.98(2.18,3.90)pg/ml和11.01(7.12,26.06)pg/ml,组间差异有统计学意义(P=0.001);两个暴露组均高于对照组,差异有统计学意义(P均<0.05),其余各因子与对照组差异无统计学意义。各组大鼠眼后段细胞组织形态无明显异常。血清中IL-1β水平组间差异无统计学意义。结论大鼠在严重污染大气及细颗粒污染空气中暴露6 d时,眼后段IL-1β的表达上调,提示短期严重空气污染可能引起眼底的炎症反应。Objective To study the expression of inflammatory factors in posterior segment of eyeball in rats exposed to shortterm severe atmospheric pollution.Methods Thirty-six healthy female SD rats with SPF grade were randomly divided into control group,air pollution group and PM2.5 group,with 12 rats in each group.The control group did not receive any exposure treatment,two exposure groups received real-time collection of polluted air or polluted air in which the particulate matters larger than 2.5μm were filtered.The exposure time was 6 days.After death,pathological changes were observed on retinal HE staining sections.Expression of IL-1β,IL-5,IL-18,MCP-1 in the posterior segment of eyes were detected using the multifactor chip method.The expression of serum IL-1βwas also detected.Results The median expressions of IL-1βin the posterior segment in control group and the other two exposed groups were 1.72(1.56,2.24)pg/ml,2.98(2.18,3.90)pg/ml,and 11.01(7.12,26.06)pg/ml,respectively,with significant difference(P=0.001),and it was significantly higher in the two exposed groups than the control group(P<0.05,respectively).While,no significant difference was found in other factors between the three groups.The posterior segment of the exposed eyes had no significant difference with the control in pathological examination,and the serum IL-1βwas not significantly different,too.Conclusion Short-term and severe air pollution with or without large particulate matters can promote the IL-1βexpression in the posterior segment of eyes,whereas,the expression of IL-5,IL-18 and MCP-1 do not change significantly.This experiment suggests that short-term and severe air pollution may cause inflammation in the fundus.

关 键 词：大气污染 细颗粒物 细胞因子 白细胞介素-1Β 眼后段 大鼠 

分 类 号：R994.6[医药卫生—毒理学] X51[医药卫生—药学]