液相色谱-串联质谱法测定饲料中喹噁啉类药物的不确定度评价  

Evaluation of Uncertainty in Determination of Quinoxalines Contained in Feed by Liquid Chromatography Tandem Mass Spectrometry

作　　者：贾铮[1] 李兰[1] 赵根龙 周剑[1] 肖志明[1] 李阳[1] 樊霞[1] JIA Zheng;LI Lan;ZHAO Genlong;ZHOU Jian;XIAO Zhiming;LI Yang;FAN Xia(Institute of Quality Standards&Testing Technology for Agro-Products,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]中国农业科学院农业质量标准与检测技术研究所,国家饲料质量监督检验中心(北京),北京100081

出　　处：《中国畜牧杂志》2020年第12期191-195,共5页Chinese Journal of Animal Science

基　　金：“十三五”国家重点研发计划项目课题(2016YFF0201105);中央级公益性科研院所基本科研业务费专项(Y2019PT01);中国农业科学院科技创新工程支持。

摘　　要：本试验旨在采用高效液相色谱-串联质谱法测定配合饲料中4种喹噁啉类药物含量,并进行不确定度来源分析和评定。考察标准溶液的配制、样品称量、样品提取、净化过程及仪器测定等主要因素引入的不确定度来源,对各不确定度进行定量分析和合成。结果表明:仪器响应值所产生的不确定度分量最大,而样品称量引入的不确定度较小;当配合饲料中卡巴氧含量为0.334 mg/kg时,其扩展不确定度为0.020 mg/kg;乙酰甲喹含量为0.342 mg/kg时,其扩展不确定度为0.018 mg/kg;喹乙醇含量为0.455 mg/kg时,其扩展不确定度为0.016 mg/kg;喹烯酮含量为0.379 mg/kg时,其扩展不确定度为0.023 mg/kg。In order to evaluate the uncertainty of determination of quinoxalines contained in feed by liquid chromatography tandem mass spectrometry,this study was conducted to examine the uncertainty of main factors such as standard solution preparation,sample weighing,sample extraction and purification,instrument analysis.The components of uncertainty were calculated and synthesized.The results indicated that the main source of uncertainty arose from the instrument analysis,on the contrary,sample weighing make little contribution to uncertainty.Furthermore,the expanded uncertainties were 0.020,0.018,0.016,0.023 mg/kg for carbdox,mequindox,olaquindox and quincetone contents of 0.334,0.342,0.455 and 0.379 mg/kg in feed(k=2),respectively.

关 键 词：液相色谱-串联质谱法 喹噁啉 不确定度 饲料 

分 类 号：S816.17[农业科学—饲料科学]