海南马樱丹黄色和粉色花中蛋白及金属离子的比较  

Comparison of Proteins and Metal Ions in the Yellow and Pink Flowers of Lantana camara L.in Hainan

作　　者：胡远南 赵志忠[2] 吴丹[2] 邱静 刘炜[1] 陈光英[1] 何文英[1] Hu Yuannan;Zhao Zhizhong;Wu Dan;Qiu Jing;Liu Wei;Chen Guangying;He Wenying(Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education,College of Chemical and Chemical Engineering,Hainan Normal University,Haikou,571158;College of Geography and Tourism,Hainan Normal University,Haikou,571158)

机构地区：[1]海南师范大学化学与化工学院,热带药用资源化学教育部重点实验室,海口571158 [2]海南师范大学地理与旅游学院,海口571158

出　　处：《分子植物育种》2020年第23期7894-7904,共11页Molecular Plant Breeding

基　　金：国家自然科学基金(21662012;41866005)资助。

摘　　要：本研究以海南产马樱丹的黄色和粉色花朵为实验样品,采用基于BPP+酚抽提法的双向电泳技术,在优化条件下,对海南马樱丹的黄花和粉花进行了提取分离,获得表达较好的蛋白质凝胶图谱,再利用Image Master 5.0软件分析凝胶图像和MALDI-TOF-MS进行酶解产物的鉴定,最后利用生物信息学手段,确定了马樱丹黄花中含有23种高表达蛋白,粉花中含有25种高表达蛋白。其中共同蛋白有9种,黄花和粉花中特有的差异蛋白分别为6种和7种。另外,利用微波消解电感耦合等离子体质谱法(ICP-MS),比较测定了马樱丹黄色和粉色花中的25种金属元素及其含量,结果表明其中含有K、Ca、Mg、Na、Al、Fe、Mn、Cu和Zn等,均以钾、铝、镁和铁的含量较高。基于以上实验结果,首次测定了马樱丹中所含植物蛋白及金属元素的种类和含量,可从所含蛋白质和金属元素角度补充说明马樱丹作为植物药的药理活性,为海南特色经济作物的进一步开发利用提供了理论指导。In this study,the yellow and pink flowers of Lantana camara L.harvested from Hainan were used as the experimental samples,two-dimensional electrophoresis(2-DE)based on BPP+phenol extraction was used to separate and extract the proteins from the yellow flower and pink flowers havested from Hainan Lantana camara L.under the optimized conditions.The gel images of the well-expressed protein were obtained,which were analyzed by using Image Master 5.0 software.Then the corresponding enzymatic hydrolysis products were identified by MALDI-TOF-MS.23 kinds of the differentially expressed proteins from the yellow flowers and 25 kinds from pink flowers of Lantana camara L.were determined by means of bioinformatics.There were 9 kinds of common proteins,6 kinds of peculiarly expressed proteins in yellow flower and 7 kinds of peculiarly expressed proteins in pink flower,respectively.In addition,a microwave digestion inductively coupled plasma mass spectrometry(ICP-MS)was performed to analyze 25 kinds of metal elements and their contents including K,Ca,Mg,Na,Al,Fe,Mn,Cu and Zn etc.The result indicated that the contents of K,Al,Mg and Fe are higher.Based on the above experimental results,it is the first time to measure the types and contents of vegetable proteins and metal elements contained in the flowers of Lantana camara L..It is possible for further explanations on its pharmacological activity as a botanical drug from the point of proteins and metal elements,which provides a theoretical guidance for the further development and utilization of Hainan characteristic economic crops.

关 键 词：马樱丹(Lantana camara L.) 差异显示蛋白 金属元素 ICP-MS 

分 类 号：S685.99[农业科学—观赏园艺]