2种SARS-CoV-2抗体检测方法在COVID-19诊断中的价值  

Roles of 2 SARS-CoV-2 antibody assays in the diagnosis of corona virus disease 2019

作　　者：胡燕[1] 申鸽 李琼 曾紫嫣 朱攀[2] 杨钢 李萍[1] 陈煜枫 张贞[1] 谢小兵[1] HU Yan;SHEN Ge;LI Qiong;ZENG Ziyan;ZHUPan;YANG Gang;LI Ping;CHEN Yufeng;ZHANG Zhen;XIE Xiaobing(Department of Clinical Pathology Laboratory,the First Hospital of Hunan University of Chinese Medicine,Changsha 410007,Hunan,China;Centers for Disease Control of Loudi,Loudi 417000,Hunan,China)

机构地区：[1]湖南中医药大学第一附属医院医学检验与病理中心,湖南长沙410007 [2]娄底市疾病预防控制中心,湖南娄底417000

出　　处：《检验医学》2020年第12期1294-1297,共4页Laboratory Medicine

基　　金：湖南省高等学校科学研究重点项目(12A10)。

摘　　要：目的探讨化学发光法和酶联免疫吸附试验(ELISA)检测严重急性呼吸综合征冠状病毒2(SARS-CoV-2)IgG抗体和IgM抗体的性能。方法分别采用化学发光法和ELISA检测116例新型冠状病毒肺炎(COVID-19)患者和346例非COVID-19者的血清SARS-CoV-2 IgG抗体和IgM抗体。结果化学发光法和ELISA检测血清SARS-CoV-2 IgG抗体的敏感性分别为90.52%、63.79%,特异性分别为98.84%、96.53%,准确性分别为96.75%、88.31%;检测血清SARS-CoV-2 IgM抗体的敏感性分别为75.86%和58.62%,特异性分别为99.42%和97.4%,准确性分别96.86%和87.66%。结论化学发光法和ELISA检测血清SARS-CoV-2 IgG抗体和IgM抗体的敏感性、特异性、准确性等均较高,临床实验室可根据实际情况选择合适的方法。Objective To determine the immunoglobulin G(IgG)and IgM antibodies to severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)by chemiluminescence and enzyme-linked immunosorbent assay(ELISA).Methods A total of 116 patients diagnosed as COVID-19 and 346 patients without COVID-19 were enrolled,and serum SARS-CoV-2 IgG and IgM antibodies were determined by chemiluminescence and ELISA,respectively.Results The sensitivities,specificities and accuracies of chemiluminescence and ELISA determining serum SARS-CoV-2 IgG were 90.52%and 63.79%,98.84%and 96.53%,96.75%and 88.31%,respectively.The sensitivities,specificities and accuracies of determining serum SARS-CoV-2 IgM were 75.86%and 58.62%,99.42%and 97.4%,96.86%and 87.66%,respectively.Conclusions The determinations of serum SARS-CoV-2 IgG and IgM antibodies by chemiluminescence and ELISA have high sensitivities,specificities and accuracies.Clinical laboratories may choose suitable method according to actual situation.

关 键 词：严重急性呼吸综合征冠状病毒2 抗体 化学发光法 酶联免疫吸附试验 新型冠状病毒肺炎 

分 类 号：R446.1[医药卫生—诊断学]