铁掺杂碳点作为过氧化物模拟酶用于谷胱甘肽检测研究  

Study on the glutathione detection by using iron-doped carbon dots as peroxidase mimetic enzyme

作　　者：孟铁宏[1] 杨湘怡 姜艳萍[1] 陶晨[1] 罗亚男[1] 任竹君[1] 李咏梅[1] 胡先运[1,2] 李春荣 MENG Tie-hong;YANG Xiang-yi;JIANG Yan-ping;TAO Chen;LUO Ya-nan;REN Zhu-jun;LI Yong-mei;HU Xian-yun;LI Chun-rong(Qiannan Medical College for Nationalities,Duyun 558000,China;The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guiyang 550002,China)

机构地区：[1]黔南民族医学高等专科学校,贵州都匀558000 [2]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550002

出　　处：《化学研究与应用》2020年第12期2133-2138,共6页Chemical Research and Application

基　　金：国家自然科学基金项目(81860317)资助;贵州省自然科学基金项目(黔科合基础[2020]1Y051,黔科合基础[2019]1301号,黔科合支撑[2019]2821号)资助;黔南州科技计划项目(黔南州科合社字[2017]19号)资助;黔南民族医学高等专科学校基金项目(QNYZ201902,QNYZ201801,QNYZ201807,QNYZ201706)资助。

摘　　要：以芦丁、尿素和三氯化铁为原料,水热法合成了铁掺杂碳点(Fe-CDs),并对制备的Fe-CDs进行了透射电子显微镜、X-射线光电子能谱和傅里叶红外光谱的表征。Fe-CDs表面富含-COOH、-OH和-NH2等含氧官能团,粒径主要分布在0.5~5 nm。Fe-CDs可催化H2O2氧化3,3',5,5'-四甲基联苯胺,并在652 nm处出现较强的吸收峰。而谷胱甘肽(GSH)能还原ox-TMB使体系吸收峰强度降低。基于上述原理,建立了以Fe-CDs为过氧化物模拟酶的GSH测定方法。GSH浓度与体系吸收峰强度的变化值(ΔA)在2~30μmol·L^-1浓度范围内呈良好的线性关系,检出限为0.8μmol·L^-1。将该方法用于测定实际样品谷胱甘肽片的含量,加标回收率在98%~103%,RSD为1.4%~2.2%。结果表明,该方法能够用于实际样品谷胱甘肽片中GSH的含量测定。Iron-doped carbon dots(Fe-CDs)was synthesized from rutin,urea and ferric chloride by hydrothermal method and was characterized by using TEM images,XPS,FTIR spectroscopy.There are rich-COOH,-OH and-NH2 groups on the surface of Fe-CDs,particle size mainly distribution in 0.5~5 nm.Fe-CDs can catalyse H2 O2 oxidation TMB reaction and there emerged a strong absorbance at 652 nm.However,GSH can reduce ox-TMB then reduce the absorbance intensity of system.Based on the above prin-ciples,a method with Fe-CDs as peroxidase mimetic enzyme for GSH detection was established.There was a good linear relationship between GSH and changes intensity of absorbance peak(ΔA)in the range from 2 to 30μmol·L^-1,the detection of limited(3σ/k)was 0.8μmol·L^-1.The content of GSH in sample was investigated with this method,the adding standard recovery was 98%~103%and RSD was 1.4%~2.2%.The results showed that this method is simple and intuitive,and can determine the content of GSH in actual samples.

关 键 词：铁掺杂碳点 过氧化物模拟酶 谷胱甘肽 

分 类 号：O657.3[理学—分析化学]