不同产地及炮制方法雪上一枝蒿中雪上一枝蒿甲素含量对比研究  

A Comparative Study on Content of Bullatine A in Aconitum brachypodum Diels.in Different Geographical Areas and Processing Methods

作　　者：牛延菲 曹红云 徐怡 张晓南 王鹏 苏钛 NIU Yan-Fei;CAO Hong-yun;XU Yi;ZHANG Xiao-nan;WANG Peng;SU Tai(School of Chemistry and Engineering,Southwest Forestry University,Yunnan Kunming 650224;Yunnan Institute of Materia Medica,Yunnan Kunming 650111,China)

机构地区：[1]西南林业大学化学与工程学院,云南昆明650224 [2]云南省药物研究所,云南昆明650111

出　　处：《广州化工》2020年第24期115-118,共4页GuangZhou Chemical Industry

基　　金：云南省重大科技专项计划-生物医药重大专项(2018ZF011)。

摘　　要：建立雪上一枝蒿中雪上一枝蒿甲素的含量测定方法,并考察不同产地、不同炮制方式对雪上一枝蒿甲素含量的影响。使用Waters Xterra MS C 18柱(5μm,4.6 mm×250 mm),以磷酸盐缓溶液和甲醇为流动相,进行梯度洗脱;流速:1.0 mL·min^-1,检测波长:210 nm。结果显示,雪上一枝蒿甲素在0.0515~10.3000μg范围内呈良好的线性关系(r=0.9999),回收率为93.57%~100.55%(n=9),RSD值为3.0%。本法准确、可靠,重现性好,适用于测定雪上一枝蒿中雪上一枝蒿甲素含量;不同产地不同批次药材中的甲素含量存在显著差异;炮制方式对雪上一枝蒿甲素含量无显著影响。A method for the determination of bullatine A content in Aconitum brachypodum Diels was established,and the influence of different geographica areas and different processing methods on the content of bullatine A in Aconitum brachypodum Diels.was investigated.The content of derivatives was determined by HPLC.HPLC was performed on Waters Xterra C 18 column(5μm,4.6 mm×250 mm),phosphate buffer solution and methanol as mobile phase,using gradient elution with flow rate of 1.0 mL·min-1,and detection wavelength of 210 nm.Bullatine A had a good linear relationship in the range of 0.0515~10.3000μg(r=0.9999),the recovery rate was 93.57%~100.55%(n=9),and RSD value was 3.0%.This method was accurate,reliable and reproducible.It was suitable for the determination of the artemisinin content of Artemisia sphaerocephala.There were significant differences in the content of artemisinin in different batches of medicinal materials from different origins.Different processing methods had no significant effect on the content of bullatine A.

关 键 词：雪上一枝蒿 雪上一枝蒿甲素 高效液相色谱 产地 炮制 

分 类 号：R917[医药卫生—药物分析学]