漆黄素对小鼠缺血再灌注肝脏炎症性损伤的保护作用  

Protection of fisetin on inflammatory liver injury induced by hepatic ischemia-reperfusion in mice

作　　者：李泽信[1] 王霄[2] 王迎[1] 李建[1] 李荣[3] 王建国[1] LI Zexin;WANG Xiao;WANG Ying;LI Jian;LI Rong;WANG Jianguo(Department of General Surgery,the First Affiliated Hospital of Xinxiang Medical University,Weihui 453100,Henan Province,China;School of Pharmacy,Xinxiang Medical University,Xinxiang 453003,Henan Province,China;School of Nursing,Xinxiang Medical University,Xinxiang 453003,Henan Province,China)

机构地区：[1]新乡医学院第一附属医院肝胆胰外科,河南卫辉453100 [2]新乡医学院药学院,河南新乡453003 [3]新乡医学院护理学院,河南新乡453003

出　　处：《新乡医学院学报》2020年第11期1018-1022,共5页Journal of Xinxiang Medical University

基　　金：新乡医学院第一附属医院青年基金资助项目(编号:QN-2017-B001)。

摘　　要：目的探讨漆黄素对小鼠缺血再灌注肝脏炎症性损伤的保护作用及其机制。方法将24只健康雄性C57BL/6小鼠随机分为假手术组、缺血再灌注组、低剂量漆黄素组和高剂量漆黄素组,每组6只。缺血再灌注组、低剂量漆黄素组和高剂量漆黄素组小鼠建立肝脏缺血再灌注模型,假手术组小鼠仅进行开关腹手术。制作模型前1 h,低剂量漆黄素组和高剂量漆黄素组小鼠分别腹腔注射漆黄素25、50 mg·kg-1;假手术组和缺血再灌注组小鼠腹腔注射等体积的二甲亚砜。缺血1 h后进行再灌注,再灌注6 h后以40 g·L-1水合氯醛腹腔注射(0.1 mL·10 g-1)麻醉小鼠,获取下腔静脉血液样本及肝脏。采用实时荧光定量聚合酶链反应法检测各组小鼠肝组织中核因子-κB(NF-κB)p65 mRNA表达,酶联免疫吸附试验检测各组小鼠肝组织中肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平及血清中丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)水平;苏木精-伊红染色法观察各组小鼠肝组织病理学变化,并计算肝组织坏死面积所占百分比。结果再灌注6 h后,假手术组、缺血再灌注组、低剂量漆黄素组和高剂量漆黄素组小鼠肝组织中NF-κB p65 mRNA相对表达量分别为1.012±0.224、6.893±0.716、3.287±0.402、1.238±0.311;缺血再灌注组小鼠肝组织中NF-κB p65 mRNA相对表达量显著高于假手术组(P<0.05),低剂量漆黄素组小鼠肝组织中NF-κB p65 mRNA相对表达量显著低于缺血再灌注组(P<0.05),高剂量漆黄素组小鼠肝组织中NF-κB p65 mRNA相对表达量显著低于低剂量漆黄素组(P<0.05)。再灌注6 h后,缺血再灌注组小鼠肝组织中TNF-α、IL-6水平和血清ALT、AST水平显著高于假手术组(P<0.05),低剂量漆黄素组小鼠肝组织中TNF-α、IL-6水平和血清ALT、AST水平显著低于缺血再灌注组(P<0.05),高剂量漆黄素组小鼠肝组织中TNF-α、IL-6水平和血清ALT、AST水平显著低于低剂量漆黄素�Objective To investigate the protection and mechanism of fisetin on the inflammatory liver injury induced by hepatic ischemia-reperfusion in mice.Methods Twenty-four C57BL/6 male mice were randomly divided into sham operation group,ischemia-reperfusion group,low-dose fisetin group and high-dose fisetin group,with six mice in each group.The hepatic ischemia-reperfusion models of mice were established in the ischemia-reperfusion group,low-dose fisetin group and high-dose fisetin group,while the mice in the sham operation group were only performed with abdominal operation.The mice in the low-dose fisetin group and high-dose fisetin group were respectively given fisetin(25,50 mg·kg-1)by intraperitoneal injection at one hour before modeling;while the mice in the sham operation group and ischemia-reperfusion group were given the same volume of dimethyl sulfoxide by intraperitoneal injection.After one hour of ischemia and six hours of reperfusion,the mice were anesthetized with 40 g·L-1 chloral hydrate by intraperitoneal injection(0.1 mL·10 g-1),and the blood samples of inferior vena cava and hepatic tissues were obtained.The expression of nuclear factor-κB(NF-κB)p65 mRNA in hepatic tissues of mice in each group was detected by real-time fluorescence quantitative polymerase chain reaction.The levels of tumor necrosis factorα(TNF-α)and interleukin-6(IL-6)in hepatic tissues of mice were measured by enzyme-linked immunosorben assay,while the levels of serum alanine transarninase(ALT)and aspartate transaminase(AST)were determined by enzyme-linked immunosorben assay.The pathological changes of hepatic tissues were observed by hematine-eosin staining,and the percentage of necrotic area of hepatic tissues was calculated.Results After six hours of reperfusion,the relative expression of NF-κB p65 mRNA in hepatic tissues of mice in the sham operation group,ischemia-reperfusion group,low-dose fisetin group and high-dose fisetin group was 1.012±0.224,6.893±0.716,3.287±0.402 and 1.238±0.311,respectively.The relative expr

关 键 词：漆黄素 缺血再灌注 肝损伤 核因子-ΚB 炎性损伤 

分 类 号：R619.9[医药卫生—外科学]