基于网络药理学研究黄芪-半枝莲药对治疗肺癌机制  

Mechanisms of the drug pair Astragali Radix-Scutellariae Barbatae Herba in treating lung cancer based on network pharmacology

作　　者：侯玥 李强[2] 王岱杰 崔莉 郑心 HOU Yue;LI Qiang;WANG Dai-jie;CUI Li;ZHENG Xin(School of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250014, China;Shandong Analysis and Test Center, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250014, China;Qingdao Haici Medical Group, Qingdao 266033, China)

机构地区：[1]山东中医药大学中医学院,山东济南250014 [2]齐鲁工业大学(山东省科学院)山东省分析测试中心,山东济南250014 [3]青岛海慈医疗集团,山东青岛266033

出　　处：《山东科学》2020年第6期34-43,共10页Shandong Science

基　　金：济南市科技发展计划(201805093);国家中医药领军人才支持计划——岐黄学者。

摘　　要：探讨黄芪-半枝莲药对治疗肺癌的机制。通过数据库检索黄芪-半枝莲有效活性成分、靶蛋白和肺癌相关疾病靶点,分别构建药物-化合物-靶点和化合物-靶点-疾病网络。构建关键靶点蛋白质-蛋白质相互作用(protein-protein interaction,PPI)网络,用R软件进行基因本体(gene ontology,GO)和京都基因和基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)的关键靶点的富集分析。得到黄芪-半枝莲药对的16个有效活性成分,作用于111个肺癌相关靶点,关键靶点包括TP53、AKT1、MAPK、TNF、VEGFA、IL-6等。GO富集分析主要涉及RNA转录、炎症、胞外和胞核,KEGG信号通路主要有TLR、NLR、HIF-1、FoxO、PI3K-Akt等信号通路,黄芪-半枝莲药对通过抑制炎症、信号转导、血管生成等过程共同起到治疗肺癌的作用。研究结果初步预测了黄芪-半枝莲药对治疗肺癌的分子机制,为该药对药理作用的深入研究奠定了基础。We aimed to determine the mechanism of Astragali Radix-Scutellariae Barbatae Herba(AR-SBH)medicine in treating lung cancer.The active ingredient and target protein of the drug pair AR-SBH and lung cancer-related disease targets were searched through a database.The drug-compound-target and compound-target-disease networks were constructed.A protein-protein interaction(PPI)network of key targets was established,followed by analysis using R software for gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)key target enrichment.Sixteen effective active ingredients of the drug pair AR-SBH were obtained,which acted on 111 lung cancer-related targets.Key targets included TP53,AKT1,MAPK,TNF,VEGFA,and IL-6.GO enrichment analysis mainly involves RNA transcription,inflammation,extracellular,and nuclear.KEGG signaling pathway mainly includes TLR,NLR,HIF-1,FoxO,PI3K-Akt,and other pathways.AR-SBH plays a role in treating lung cancer by inhibiting inflammation,signal transduction,angiogenesis,and other processes.The results predict the molecular mechanism of AR-SBH in lung cancer treatment and lay a foundation for further in-depth study on the pharmacological effects of the drug pair AR-SBH.

关 键 词：黄芪-半枝莲 肺癌 网络药理学 炎症 

分 类 号：R285[医药卫生—中药学]