表面增强拉曼光谱对抗肿瘤药物5-氟尿嘧啶的检测研究  

Detection of 5-Fluorouracil by Surface Enhanced Raman Spectroscopy

作　　者：周国良 黄光耀 李盼 王宏志[1,3] 杨良保 ZHOU Guoliang;HUANG Guangyao;LI Pan;WANG Hongzhi;Yang Liangbao(Center of Medical Physics and Technology,Hefei Institutes of Physical Science,Chinese Academy of Sciences,Hefei 230031,China;University of Science&Technology of China,Hefei 230026,China;Cancer Hospital,Chinese Academy of Sciences,Hefei 230031,China)

机构地区：[1]中国科学院合肥物质科学研究院医学物理与技术中心,安徽合肥230031 [2]中国科学技术大学,安徽合肥230026 [3]中国科学院合肥肿瘤医院,安徽合肥230031

出　　处：《光散射学报》2020年第3期224-229,共6页The Journal of Light Scattering

基　　金：国家科技重大专项“重大新药创制”项目(201ZX09J18112);安徽省自然科学基金(1908085QB65);安徽省重点研究与开发计划科技强警(1804d08020308,201904d07020009)。

摘　　要：本文利用表面增强拉曼光谱技术(SERS)实现了抗肿瘤药物5-氟尿嘧啶(5-FU)的灵敏检测。通过种子生长法制备不同粒径的银纳米颗粒(Ag NPs),利用结晶紫(CV)作为探针分子评估基底的增强能力。将优化的Ag NPs作为SERS基底,结合便携式拉曼光谱仪器,对不同浓度的5-氟尿嘧啶(5-FU)标准溶液进行检测,考察其检测限。最终利用标准加入法测定人体血清中5-FU。通过有机相沉淀去除蛋白质,降低检测干扰。结果表明,血清中5-FU检测限为3.125 g/mL。In this paper,Surface Enhanced Raman Spectroscopy(SERS)was used to realize the sensitive detection of 5-fluorouracil(5-FU).Silver nanoparticles(AgNPs)with different diameters were prepared by seed growth method.Crystal Violet(CV)was used as a probe molecule to evaluate the enhancement ability of the SERS substrate.The optimized AgNPs were used as SERS substrates and the standard solutions of 5-FU with different concentrations were detected by portable Raman spectroscopy.Finally,5-FU spiked in human serum was determined after the pretreatment of serum.Protein was removed by organic precipitation to reduce detection interference and the detection limit of 5-FU in serum was 3.125 g/mL.

关 键 词：抗肿瘤药物 5-氟尿嘧啶 表面增强拉曼光谱 银纳米颗粒 

分 类 号：O657.37[理学—分析化学]