LvCTL1与WSSV复制的相互关系研究  

The Relationship between LvCTL1 and WSSV Replication

作　　者：邱炜 陈义烘 QIU Wei;CHEN Yihong(School of Biology and Engineering,Guizhou Medical University,Guiyang 550025,China;School of Life Sciences,South China Normal University,Guangzhou 510631,China)

机构地区：[1]贵州医科大学生物与工程学院,贵阳550025 [2]华南师范大学生命科学学院,广州510631

出　　处：《华南师范大学学报（自然科学版）》2020年第6期52-56,共5页Journal of South China Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(31960206,31772895);贵州省自然科学基金项目(J-[2020]1Y334)。

摘　　要：C型凝集素(CTL)是一类重要的先天性免疫因子,在对虾机体的免疫防御中具有重要的作用.该研究利用相对定量PCR实验检测基因表达量、绝对定量PCR实验检测病毒拷贝数、组织切片分析实验检测感染病毒细胞数.实验数据显示:WSSV感染能显著上调LvCTL1的表达;WSSV感染的LvCTL1敲降的凡纳滨对虾中,膜囊蛋白VP28表达量、病毒拷贝数和胃上皮细胞中的感染细胞数都显著高于注射dsEGFP对照组.研究结果表明:WSSV感染后,机体通过上调LvCTL1表达来抑制WSSV复制.C-type lectin(CTL)is an important innate immune factor that plays an important role in the immune defense of prawns.The gene expression was detected with relative quantitative PCR,the virus number was detected with absolute quantitative PCR,and the number of infected cells was detected with histology analysis.The data showed that the expression of LvCTL1 was significantly upregulated with WSSV challenge;the expression of WSSV envelope protein VP28,virus loads and the number of infected cells of stomach epithelium in LvCTL1 knockdown prawns upon WSSV infection were significantly higher than those under the dsEGFP control.These results indicated that LvCTL1 expression was significantly activated with WSSV challenge and played a role in WSSV replication inhibition.

关 键 词：C型凝集素 白斑综合症病毒 凡纳滨对虾 

分 类 号：TN27[电子电信—物理电子学]