联合检测人乳头病毒与单纯疱疹病毒在宫颈病变筛查中的价值  

Value of Joint Detection of Human Papillomavirus(HPV)and Herpes Simplex Virus(HSV-Ⅱ)in Cervical Lesions Screening

作　　者：霍中华[1] 李明成 HUO Zhonghua;LI Mingcheng(Affiliated Hospital of Beihua Universtiy,Jilin 132011,China;Medical Technology College of Beihua University,Jilin 132013,China.)

机构地区：[1]北华大学附属医院,吉林吉林132011 [2]北华大学医学技术学院,吉林吉林132013

出　　处：《北华大学学报（自然科学版）》2020年第6期779-782,共4页Journal of Beihua University（Natural Science）

基　　金：吉林省教育厅科学技术研究项目(2013172);吉林省卫生和计划生育委员会科学技术研究项目(2014Z077).

摘　　要：目的探讨联合检测人乳头病毒(HPV)与单纯疱疹病毒(HSV-Ⅱ)在宫颈病变筛查中的价值.方法收集已婚女性宫颈标本1269例,行液基波层细胞学检测,根据检测结果分为轻微炎症组、低级别病变组(宫颈上皮内瘤变(cervical intraepi-thelial-neoplasia,CIN)Ⅰ)、高级别病变组(CINⅡ~Ⅲ)及癌变组(浸润性宫颈癌(invasive-cervicalcancers,ICC));采集同期健康体检中心妇科体检已婚女性128例宫颈标本为正常对照组.PCR-反向点杂交法进行HPV核酸检测及基因分型;PCR-荧光探针法检测HSV-Ⅱ核酸.结果宫颈病变患者中HPV总检出率为63.1%,高于正常对照组(12.5%)(P<0.05);HSV-Ⅱ检出率为28.9%,高于正常对照组(6.3%)(P<0.05).HPV高危型中25岁(包括25岁)以下和46~55年龄组的感染率高;而HPV低危型在46~55年龄组的感染率高,与其他各年龄段比较差异均具有统计学意义(P<0.05).HSV-Ⅱ感染率与HPV高危型感染率年龄段相似,25岁(包括25岁)以下和46~55年龄组的感染率高,与其他各年龄段比较差异具有统计学意义(P<0.05).HPV与HSV-Ⅱ混合感染检出率为14.9%,46~55年龄组的感染率高,与其他各年龄段比较差异均具有统计学意义(P<0.05).经Spearman相关性分析,宫颈病变严重程度与患者HVP和HSV-Ⅱ感染呈正相关(r=0.536,P<0.05);随着宫颈病变程度的加重,HPV、HSV-Ⅱ及HPV与HSV-Ⅱ混合感染率都明显增加,各组间HPV、HSV-Ⅱ的感染率差异具有统计学意义(P<0.05).结论HPV与HSV-Ⅱ在宫颈病变患者中感染率较高,在临床中有必要加强早期宫颈病变患者HPV与HSV感染的检测,并给予抗病毒治疗.Objective To explore the value of joint detection of human papillomavirus(HPV)and herpes simplex virus(HSV-Ⅱ)in screening for cervical lesions.Method 1269 specimens were collected from the married women with cervical erosion,were classified into four groups including mild inflammation group,lowgrade lesions group,high-grade lesions group and invasive-cervical cancers group based on the TCT(thinprep cytologic test).In the same period,128 cases of cervix specimens from married women were collected in the gynecology health examination center of the hospital as a normal control group.The fluorescence quantitative PCR and PCR-reverse dot hybridization were used to detect HPV,subtypes and HSV-Ⅱ respectively.Results The positive for HPV was 63.1%and higher than that of health control groups(12.5%)(P<0.05).The positive for HSV-Ⅱ was 28.9%and higher than that of health control groups(6.3%)(P<0.05).Among of all patients,highrisk HPV was distributed in groups of under 25-year and 46~55-year,whereas,low-risk HPV was susceptible for the age of 46~55-year.The positive rate of HSV-Ⅱ was the same as the high-risk HPV in ages.Besides,the mixed infection of HPV and HSV-Ⅱ was 14.9%,and distributed in groups of 46~55-year(P<0.05).Spearman correlation analysis proved that the HPV infection in the patients with cervical erosion was positively correlated with the HSV-Ⅱ infection(r=0.536,P<0.05).With the aggravation of cervical lesions,the HPV,HSV-Ⅱ and the mixed infection rate of HPV and HSV-Ⅱ increased significantly,and the difference of HPV and HSV-Ⅱ infection rates between the groups was statistically significant(P<0.05).Conclusion The infection rate of HPV and HSV-Ⅱ were higher in patients with cervical erosion in the region,it is essential to strengthen the cervical erosion patients with HPV and HSV infection detection and to take early measures to antiviral therapy in clinic.

关 键 词：宫颈病变 人乳头病毒(HPV) 单纯疱疹病毒(HSV-Ⅱ) 共同感染 

分 类 号：R711.32[医药卫生—妇产科学]