神香草对哮喘小鼠体内Tfh细胞的改善作用  

Improvement of Tfh cells in asthmatic mice by Hyssopus officinalis L.

作　　者：马小娟[1] 马志兴 于文燕[1] 周宁 丁剑冰[4] 孙湛[1] MA Xiaojuan;MA Zhixing;YU Wenyan;ZHOU Ning;DING Jianbing;SUN Zhan(Department of Pathophysiology,College of Basic Medicine,Xinjiang Medical University,Urumqi 830054,China;Emergency Department,General Hospital of Xinjiang Military Command,Urumqi 830000,China;Department of Gastroenterology,The Affiliated Cancer Hospital of Xinjiang Medical University,Urumqi 830054,China;Department of Immunology,College of Basic Medicine,Xinjiang Medical University,Urumqi 830054,China)

机构地区：[1]新疆医科大学基础学院病理生理学教研室,新疆乌鲁木齐830054 [2]新疆军区总医院急诊科,新疆乌鲁木齐830000 [3]新疆医科大学第三临床医学院消化内科,新疆乌鲁木齐830054 [4]新疆医科大学基础学院免疫学教研室,新疆乌鲁木齐830054

出　　处：《西部医学》2020年第12期1744-1748,共5页Medical Journal of West China

基　　金：新疆维吾尔自治区自然科学基金(2019D01C187)。

摘　　要：目的观察神香草预处理对哮喘小鼠的保护作用,并探讨其可能机制。方法将32只Balb/c小鼠随机分为对照组、哮喘组、地塞米松组及神香草组4组,每组8只。除对照组外,其余3组制备哮喘模型。哮喘组于第1天、第15天腹腔注射0.2 mL致敏液,第22天雾化吸入1%卵清蛋白(OVA)溶液,每次30 min,每周3次,连续8周。地塞米松组和神香草组分别于OVA激发前1 h给予地塞米松0.005 mg/10 g和神香草提取液0.04 g/10 g灌胃,每日1次,连续8周。对照组以生理盐水代替OVA处理。8周后,取材,流式细胞术检测外周血中CD4^+CXCR5^+/CD4^+和CD4^+CXCR5^+ICOS^+/CD4^+CXCR5^+的比例,Real-time PCR检测各组肺组织中BCL-6、CXCR5、ICOS、ICOSL和IL-21 mRNA的表达,并观察肺组织病理学改变。结果与哮喘组比较,神香草组外周血CD4^+CXCR5^+/CD4^+和CD4^+CXCR 5^+ICOS^+/CD4^+CXCR5^+的比例减少,并且肺组织中BCL-6、CXCR5、ICOS、ICOSL和IL-21 mRNA的表达亦明显降低(均P<0.05)。结论神香草可能通过减少Tfh细胞的数量和影响其相关分子的表达而在慢性哮喘治疗中发挥作用。Objective To observe the protective effect of Hyssopus officinalis L.pretreatment on asthmatic mice and explore its possible mechanism.Methods Thirty two Balb/c mice were randomly divided into 4 groups:control group,asthma group,dexamethasone group and Hyssopus officinalis L.group,with 8 mice in each group.Except for the control group,the other three groups made asthma models.In asthma group,0.2 ml sensitizing solution was injected intraperitoneally on the 1st and 15th days,and 1%ova solution was inhaled on the 22nd day,30 minutes each time,three times a week for 8 weeks.Dexamethasone(0.005mg/10g)and extract(0.04g/10g)were given by gavage one hour before OVA challenge in dexamethasone group and shenvanilla group,respectively,once a day for 8 weeks.The control group was treated with normal saline instead of ova.After 8 weeks,samples were taken and the expression of BCL-6,CXCR5,ICOS,ICOSL and IL-21 mRNAs in lung tissues were detected by real time PCR.Results Compared with the asthmatic group,the percentage of CD4^+CXCR5^+/CD4^+,CXCR5^+ICOS^+/CD4^+CXCR5^+,ICOS^+/CD4^+CXCR5^+and the expressions of BCL-6,CXCR5,ICOS,ICOSL and IL-21 mRNA in lung tissues were significantly decreased in Hyssopus officinalis L.group(P<0.05).Conclusion Hyssopus officinalis L.may play a therapeutic role in chronic asthma by reducing the number of Tfh cells and affecting the expression of related molecules.

关 键 词：神香草 哮喘 Tfh细胞 

分 类 号：R562.25[医药卫生—呼吸系统]