19个自育葡萄品种(系)指纹图谱构建  

Construction of the Fingerprints of 19 Self-bred Grape(Vitis vinfera)Varieties(Lines)

作　　者：王勇 孙锋 苏来曼·艾则孜 李玉玲 伍国红 骆强伟 郭平峰 WANG Yong;SUN Feng;Sulaiman Aizezi;LI Yuling;WU Guohong;LUO Qiangwei;GUO Pingfeng(Research Institute of Grapes and Melons of Xinjiang Uygur Autonomous Region, Shanshan Xinjiang 838200, China)

机构地区：[1]新疆维吾尔自治区葡萄瓜果研究所,新疆鄯善838200

出　　处：《新疆农业科学》2020年第12期2238-2249,共12页Xinjiang Agricultural Sciences

基　　金：现代农业产业技术体系建设专项基金(CARS-29-yc-7);新疆维吾尔自治区公益性科研院所基本科研业务经费项目(KY2019066);新疆维吾尔自治区重点研发任务专项(2016B01005-4)。

摘　　要：【目的】利用SSR标记技术开展19个葡萄品种(系)的指纹图谱构建,为种苗认证、品种登记和植物新品种保护提供依据。【方法】以19个葡萄品种(系)为试材,利用16对SSR标记荧光引物,对其进行PCR扩增和毛细管电泳,获得指纹条带,构建指纹图谱,并筛选可以将19个品种(系)区分的引物组合,绘制种质分子身份证数字码。【结果】从16对SSR标记引物选择了VRZAG67、VVS2、VVMD7、VCHR4a、VVMD5等5对多态性信息量相对较高的SSR标记,完成了品种(系)的指纹图谱构建,并对其进行了赋值编码,形成了身份识别码。【结论】构建的指纹图谱和分子身份证可以鉴定和区别19个品种(系),但由于葡萄种质较多,如果继续区别更多的品种,需要增加的SSR引物数量。【Objective】To construct the fingerprints of 19 grape varieties(lines)by SSR technology in the hope of providing basis for seedling certification,variety registration and new plant variety protection.【Method】In this project,19 grape varieties(lines)were used as test materials,16 pairs of SSR labeled silver primers were used for PCR amplification and capillary electrophoresis to obtain the fingerprint bands,construct the fingerprint map,and select the primer combinations that could distinguish the 19 varieties(lines),encode the alleles and establish the germplasm molecular identity card.【Result】In this study,VRZAG67,VVS2,VVMD7,VCHR4a and VVMD5 were selected from 16 pairs of SSR markers,and 5 pairs of SSR markers with relatively high amount of polymorphism information were constructed.The fingerprints of varieties(lines)were constructed,and their assigned values were coded to form an identification code.【Conclusion】The fingerprints and molecular ID cards constructed can identify and distinguish 19 varieties(lines),but because there are many grape germplasms,if we continue to distinguish more varieties,we needed to increase the number of SSR primers.

关 键 词：葡萄品种 SSR标记 指纹图谱 

分 类 号：S663.1[农业科学—果树学] S188[农业科学—园艺学]