A 2-kb Mycovirus Converts a Pathogenic Fungus into a Beneficial Endophyte for Brassica Protection and Yield Enhancement  

作　　者：Hongxiang Zhang Jiatao Xie Yanping Fu Jiasen Cheng Zheng Qu Zhenzhen Zhao Shufen Cheng Tao Chen Bo Li Qianqian Wang Xinqiang Liu Binnian Tian David B.Collinge Daohong Jiang 

机构地区：[1]State Key Laboratory of Agricultural Microbiology,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,Hubei Province 430070,China [2]The Provincial Key Lab of Plant Pathology of Hubei Province,Huazhong Agricultural University,Wuhan,China [3]Department of Plant and Environmental Sciences,University of Copenhagen,1871 Frederiksberg C,Denmark

出　　处：《Molecular Plant》2020年第10期1420-1433,共14页分子植物（英文版）

基　　金：the National Science Foundation of China(31430070);the National Key R&D Program(2017YFD0200602);the China Agriculture Research System(CARS-12).

摘　　要：Mycoviruses are viruses that infect fungi,and hypovirulence-associated mycoviruses have the potential to control fungal diseases.However,it is unclear how mycovirus-mediated hypovirulent strains live and survive in the field,and no mycovirus has been applied for field crop protection.In this study,we found that a previously identified small DNA mycovirus(SsHADV-1)can convert its host,Sclerotinia sclerotiorum,from a typical necrotrophic pathogen to a beneficial endophytic fungus.SsHADV-1 downregulates the expression of key pathogenicity factor genes in S.sclerotiorum during infection.When growing in rapeseed,the SsHADV-1-infected strain DT-8 significantly regulates the expression of rapeseed genes involved in defense,hormone signaling,and circadian rhythm pathways.As a result,plant growth is promoted and disease resistance is enhanced.Field experiments showed that spraying DT-8 at the early flowering stage can reduce the disease severity of rapeseed stem rot by 67.6%and improve yield by 14.9%.Moreover,we discovered that SsHADV-1 could also infect other S.sclerotiorum strains on DT-8-inoculated plants and that DT-8 could be recovered from dead plants.These findings suggest that the mycoviruses may have the ability to shape the origin of endophytism.Our discoveries suggest that mycoviruses may influence the origin of endophytism and may also offer a novel strategy for disease control in which mycovirus-infected strains are used to improve crop health and release mycoviruses into the field.

关 键 词：MYCOVIRUS SCLEROTINIA sclerotiorum ENDOPHYTE RAPESEED stem rot biological control BRASSICA NAPUS 

分 类 号：Q94[生物学—植物学]