Nematode-Encoded RALF Peptide Mimics Facilitate Parasitism of Plants through the FERONIA Receptor Kinase  

作　　者：Xin Zhang Huan Peng Sirui Zhu Junjie Xing Xin Li Zhaozhong Zhu Jingyuan Zheng Long Wang Bingqian Wang Jia Chen Zhenhua Ming Ke Yao Jinzhuo Jian Sheng Luan Devin Coleman-Derr Hongdong Liao Yousong Peng Deliang Peng Feng Yu 

机构地区：[1]State Key Laboratory of Chemo/Biosensing and Chemometrics,College of Biology,Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation,Hunan University,Changsha 410082,P.R.China [2]State Key Laboratory of Plant Disease and Insect Pest,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,P.R.China [3]State Key Laboratory of Hybrid Rice,Hunan Hybrid Rice Research Center,Changsha 410125,P.R.China [4]Institute of Vegetable Research,Hunan Academy of Agricultural Sciences,Changsha 410125,P.R.China [5]State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,College of Life Science and Technology,Guangxi University,Nanning 530004,P.R.China [6]Department of Plant and Microbial Biology,University of California,Berkeley,CA 94720,USA

出　　处：《Molecular Plant》2020年第10期1434-1454,共21页分子植物（英文版）

基　　金：the National Natural Science Foundation of China(NSFC-31871396,31571444,31400232,and 31672012);the Young Elite Scientist Sponsorship Program from CAST(YESS20160001);the Science and Technology Inn ovation Project of Chinese Academy of Agricultural Sciences(2060302-51).

摘　　要：The molecular mechanism by which plants defend against plant root-knot nematodes(RKNs)is largely unknown.The plant receptor kinase FERONIA and its peptide ligands,rapid alkalinization factors(RALFs),regulate plant immune responses and cell expansion,which are two important factors for successful RKN parasitism.In this study,we found that mutation of FERONIA in Arabidopsis thaliana resulted in plants showing low susceptibility to the RKN Meloidogyne incognita.To identify the underlying mechanisms associated with this phenomenon,we identified 18 novel RALF-likes from multiple species of RKNs and showed that two RALF-likes(i.e.,MiRALF1 and MiRALF3)from M.incognita were expressed in the esophageal gland with high expression during the parasitic stages of nematode development.These nematode RALF-likes also possess the typical activities of plant RALFs and can directly bind to the extracellular domain of FERONIA to modulate specific steps of nematode parasitism-related immune responses and cell expansion.Genetically,both MiRALF1/3 and FERONIA are required for RKN parasitism in Arabidopsis and rice.Collectively,our study suggests that nematode-encoded RALFs facilitate parasitism via plant-encoded FERONIA and provides a novel paradigm for studying host-pathogen interactions.

关 键 词：RALF FERONIA ROOT-KNOT nematodes Arabidopsis THALIANA DISULFIDE BONDS 

分 类 号：Q94[生物学—植物学]