甘麦大枣汤对焦虑型肠易激综合征大鼠行为学和肠组织中5-HT、SP、CGRP、TNF-α的影响研究  

Effect of Ganmai Dazao Decoctions on Behavior and Intestinal Tissue 5-HT,SP,CGRP and TNF-αLevels in Rats with Irritable Bowel Syndrome

作　　者：朱静[1] 孙峰[2] 朱华[1] 甘瑜 陈旭 ZHU Jing;SUN Feng;ZHU Hua;GAN Yu;CHEN Xu(Subei Peoples Hospital of Jiangsu Province,Yangzhou 225001;Medical College of Yangzhou University,Yangzhou,225001;College of Pharmacy,Dalian Medical University,Dalian 116044)

机构地区：[1]江苏省苏北人民医院,扬州225001 [2]扬州大学医学院,扬州225001 [3]大连医科大学药学院,大连116044

出　　处：《药学与临床研究》2020年第6期408-412,共5页Pharmaceutical and Clinical Research

基　　金：江苏省中医药局科技项目(LZ13196)。

摘　　要：目的:研究甘麦大枣汤对焦虑型肠易激综合征大鼠5-HT、SP、CGRP和TNF-α含量以及肠组织病理性影响。方法:使用SD大鼠70只,除空白对照组外,各组均以噪音刺激、足底电击、夹尾刺激、束缚和番泻叶提取物灌胃方法,建立焦虑型肠易激综合征大鼠模型,模型随机分5组维持刺激并分别给予相应药物灌胃,连续用药15 d后考察行为学变化和各种IBS大鼠的肠推动,然后处死各实验组大鼠,检测大鼠结肠组织HE染色的病理学变化,使用ELISA试剂盒检测各组大鼠结肠和血清中5-HT、SP、CGRP和TNF-α含量。结果:与模型组相比,甘麦大枣汤能显著降低IBS大鼠焦虑样,但降低肠推动作用较阳性药对照组弱。甘麦大枣汤可降低肠组织和血清中的5-HT、SP和CGRP水平(P<0.05),其中高剂量组最明显(P<0.01),但各给药组中的TNF-α相比IBS模型无显著差异。病理分析显示,高剂量组和中剂量组可明显减少IBS大鼠肠组织中炎性反应。结论:甘麦大枣汤对焦虑型肠易激综合征大鼠有治疗效果,其作用机制可能与调节5-HT、SP、CGRP表达有关,对TNF-α相关因子作用不显著。Objective:To study the effects of Ganmai Dazao Decoctions on 5-HT,SP,CGRP and TNF-αcontents and intestinal histopathology in rats with irritable bowel syndrome(IBS)and anxiety.Methods:Seventy SD rats were used.Except the blank control group,anxiety-type IBS was established by noise,plantar shock,tail-clamp,restraint and senna extracts infusion in each group.The modeled rats were randomly divided into 5 groups with model stimulations maintained.After 15 days of continuous preset drug administrations,the changes behavior and intestinal motility were investigated.The pathological changes of HE staining in colon tissues were measured.Elisa kit methods were used to detect the levels of 5-HT,SP,CGRP and TNF-αin the colon and serum of rats in each group.Results:Compared with the model group,Ganmai Dazao Decoctions significantly reduced the anxiety behavior of IBS rats,but the effect of reducing intestinal pushing was weaker than that of the positive control group.Ganmai Dazao Decoctions reduced the levels of 5-HT,SP and CGRP in intestinal tissues and serum(P<0.05),which was most obvious in the high-dose group(P<0.01).There was no significant difference in TNF-αlevels between the drug administration groups and the IBS model group.Pathological analysis showed that Ganmai Dazao Decoctions at high-and middle-dose could significantly reduce the edema response in the intestinal tissues of IBS rats.Conclusion:Ganmai Dazao Decoctions have therapeutic effects on rats with irritable bowel syndrome and anxiety.The mechanism may be mainly related to the regulation of 5-HT,SP and CGRP expression.

关 键 词：甘麦大枣汤 肠易激综合征 焦虑 5-HT SP CGRP TNF-Α 

分 类 号：R965.1[医药卫生—药理学]