割手密CDPK基因家族的鉴定及表达分析  

Identification and Expression Analysis of CDPK Gene Family in Saccharum spontaneum

作　　者：张南南 黄咏虹 綦鹏 冯晓敏 吴自林 曾巧英 齐永文 ZHANG Nan-Nan;HUANG Yong-Hong;QI Peng;FENG Xiao-Min;WU Zi-Lin;ZENG Qiao-Ying;QI Yong-Wen(Guangdong Sugarcane Genetic Improvement Engineering Center/Institute of Bioengineering,Guangdong Academy of Sciences,Guangzhou 510316,China)

机构地区：[1]广东省科学院生物工程研究所/广东省甘蔗遗传改良工程中心,广州510316

出　　处：《农业生物技术学报》2020年第12期2151-2164,共14页Journal of Agricultural Biotechnology

基　　金：广东省科学院研究所绩效考核引导专项(2020GDASYL-20200302005);国家自然科学基金(31901512);广东省重点领域研发计划(2019B020238001)。

摘　　要：钙依赖蛋白激酶(calcium dependent-protein kinase,CDPKs或CPKs)在植物细胞内Ca2+信号识别和转导中具有重要作用,参与植物生长发育、对生物胁迫和非生物胁迫的抗性反应等。至今尚无甘蔗属(Saccharum)植物中CDPK基因的相关报道。本研究根据已公布的甘蔗属割手密(Saccharum spontaneum)基因组信息,利用生物信息学方法对割手密中CDPK基因家族成员进行鉴定,共鉴定到90个SsCDPKs,并对其生理生化参数、染色体定位、基因复制事件、系统进化关系、基因结构、保守基序和功能结构域等进行分析。共线性分析结果显示,有些SsCDPK基因可能与水稻(Oryza sativa)的CDPK基因来自同一祖先。进化选择压力分析发现,割手密CDPK基因在进化过程中受正向选择,可能在植物进化中具有重要作用。基于转录组数据分析SsCDPK表达特性,发现SsCDPK表达具有明显的组织和发育时期特异性,甘蔗栽培品种’粤糖55’(YT55)中部分SsCDPK基因表达受低氮和低钾胁迫的调控。本研究结果提示,SsCDPKs基因可能参与甘蔗属植物的生长发育和对营养胁迫的应答反应。本研究为深入解析CDPK基因在甘蔗属植物生长发育及对营养胁迫应答反应中的功能提供参考依据。Calcium-dependent protein kinases(CDPKs or CPKs)function as Ca2+sensors and effectors to directly translate Ca2+signals into downstream phosphorylation signals.CDPKs are involved in plant growth and developmental processes,as well as responses to abiotic and biotic stresses.However,little information is available about the CDPK family in Saccharum plants.In this study,based on the published Saccharum spontaneum genome data,the CDPK gene family members of S.spontaneum were identified by bioinformatics methods,and a total of 90 CDPKs were identified,whose physical and chemical parameters,chromosomal locations,gene duplication,phylogenetic relationships,evolutionary characteristics,gene structure,and motif distribution were analyzed.Syntenic analysis showed that consensuses in CDPKs might have existed before the species divergence between S.spontaneum and Oryza sativa.The selection pressure analysis showed that CDPK genes in S.spontaneum were positively selected in the process of evolution and might play important roles in the evolution of plants.Transcriptome analysis showed that most SsCDPKs exhibited different expression levels in different tissues and developmental stages.In addition,many SsCDPKs in sugarcane hybrid variety Yuetang 55(YT55)exhibited different expression profile under low-potassium and low-nitrogen treatments.The diverse expression of SsCDPKs suggested potential important function of SsCDPKs in regulating the development and responses to low-potassium and low-nitrogen stresses of Saccharum plants.This study provides a reference for further analysis of roles of Saccharum CDPK genes in the development and responses to nutrition stresses.

关 键 词：甘蔗属 钙依赖蛋白激酶(CDPK) 生物信息学分析 生长发育 营养胁迫 

分 类 号：S184[农业科学—农业基础科学]