吉林省西瓜蔓枯病病原鉴定及室内药剂筛选  

Pathogen Identification and Screening of the Fungicides of Citrullus lanatus Gummy Stem Blight in Jilin Province

作　　者：陈悦 孙东晗 孙于淼 赵廷昌[3] 白庆荣 CHEN Yue;SUN Donghan;SUN Yumiao;ZHAO Tingchang;BAI Qingrong(College of Plant Protection,Jilin Agricultural University,Changchun,Jilin 130118;Agricultureal and Rural Service Center in Heihe,Heihe Heilongjiang 164300;Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193)

机构地区：[1]吉林农业大学植物保护学院,吉林长春130118 [2]黑河市农业农村服务中心,黑龙江黑河164300 [3]中国农业科学院植物保护研究所,北京100193

出　　处：《北方园艺》2020年第23期16-23,共8页Northern Horticulture

基　　金：国家重点研发资助项目(2018YFD0201300);国家西甜瓜产业技术体系资助项目(CARS-25)。

摘　　要：以发病西瓜叶片为试材,采用组织分离法对采自吉林省7个市县的西瓜蔓枯病菌进行分离和致病性测定。并采用菌丝生长速率法测定了不同产区西瓜蔓枯病菌代表菌株对24种杀菌剂的敏感性,以期筛选出对防治西瓜蔓枯病的有效药剂。结果表明:通过形态学特征及三重PCR序列分析,共获得334株西瓜蔓枯病菌,332株为Stagonosporopsis citrull,为优势种,2株为S.caricae;不同地区代表菌株对相同药剂的敏感性有轻微差异;病菌对450 g·L^-1咪鲜胺EW等16种杀菌剂表现出较高的敏感性,EC50<20.0 mg·L^-1。Pathogens which caused Citrullus lanatus leaves were gotten by tissue isolated method.Species identification of Citrullus lanatus gummy stem blight in 7 cities and counties of Jilin Province was done by pathogenicity,and the susceptibility of the pathogen representative strains in different areas to 24 fungicides was tested to determine effective treatments for controlling Citrullus lanatus gummy stem blight by the method of mycelia growth rate.The results showed that morphology and triple PCR.334 strains of Stagonosporopsis spp.were obtained,including 332 dominant strains of S.citrulli,and only two strains of S.caricae.the sensitivity of representative strains from different area to the same fungicides was slight different significantly.The pathogen was high sensitive to 16 fungicides including 450 g·L^-1 prochloraz EW,their EC50 value lower than 20.0 mg·L^-1.

关 键 词：西瓜 蔓枯病 病原鉴定 药剂敏感性 

分 类 号：S651[农业科学—果树学]