解淀粉芽孢杆菌TF28对棚室连作黄瓜根际土壤真菌多样性的影响  

Effects of Bacillus amyloliquefaciens TF28 on Diversity of Rhizosphere Soil Fungi in Greenhouse Continuous Cropping Cucumber

作　　者：曹旭[1,2] 张淑梅[1,2] 李晶[1,2] 孟利强[1,2] 胡基华[1] CAO Xu;ZHANG Shumei;LI Jing;MENG Liqiang;HU Jihua(Institute of Microbiology,Heilongjiang Academy of Sciences,Harbin,Heilongjiang 150010;Institute of Advanced Technology,Heilongjiang Academy of Sciences,Harbin,Heilongjiang 150020)

机构地区：[1]黑龙江省科学院微生物研究所,黑龙江哈尔滨150010 [2]黑龙江省科学院高技术研究院,黑龙江哈尔滨150020

出　　处：《北方园艺》2020年第23期88-95,共8页Northern Horticulture

基　　金：黑龙江省院所基本应用技术研究专项资助项目(ZNBZ2018SW02)。

摘　　要：以微生物菌剂解淀粉芽孢杆菌TF28为试材,以液体(L)、颗粒(P)、复合(LP)3种方法进行施用,与不施用TF28土壤样品(CK)进行比较。取苗期、花期、盛果期和结果后期土壤样品,采用高通量测序技术,观测土壤真菌多样性和群落组成,以期改善棚室黄瓜因连作产生的土壤问题。结果表明:子囊菌门(Ascomycoba)、被孢霉门(Mortierellomycota)、担子菌门(Basidiomycota)、乳糜霉菌门(Chytridiomycota)为主要菌门,子囊菌门为主要优势菌类群。TF28可以减少真菌序列量;对镰刀菌属(Fusarium)、核盘菌属(Sclerotinia)、顶襄壳属(Gaeumannomyces)和腐霉菌属(Rhizoctonia)具有抑制作用,P处理可以提高毛壳菌属丰度,对棚室连作黄瓜土壤调节相对稳定。该研究为改善棚室连作黄瓜障碍,实现设施蔬菜持续稳定健康生产提供参考依据。Three methods,liquid(L),particle(P) and composite(LP) of the Bacillus amyloliquefaciens TF28 were used as a microbial agent.The soil samples at seedling,flowering,full fruit and later fruit bearing stage were taken and high-throughput sequencing technology was used.The results showed that Ascomycoba,Mortierellomycota,Basidiomycota and Chytridiomycota were the main phylum,Ascomycota was the main dominant group.TF28 reduced the number of fungi sequence.It could inhibit Fusarium,Sclerotinia,Gaeumannomyces and Rhizoctonia.P treatment could improve the abundance of Chaetomiumhas and relatively stable soil regulation on greenhouse Continuous Cropping Cucumber.The study provided a reference for improving cucumber barrier in greenhouse and achieving sustainable and stable and healthy production of vegetable facilities.

关 键 词：高通量测序 连作障碍 真菌丰度 施用方式 

分 类 号：S19[农业科学—农业基础科学]