多核苷酸激酶定量检测方法的研究进展  

Research progress for quantitative detection of polynucleotide kinases

作　　者：代丽雁 刘增臣 姚帅 朱文平 DAI Liyan;LIU Zengchen;YAO Shuai;ZHU Wenping(College of Chemistry and Chemical Engineering,Zhoukou Normal University,Zhoukou 466001,China)

机构地区：[1]周口师范学院化学化工学院,河南周口466001

出　　处：《周口师范学院学报》2020年第5期40-44,135,共6页Journal of Zhoukou Normal University

基　　金：国家自然科学青年基金项目“近红外双光子无机-有机杂化超分子自组装荧光探针的构建及汞离子识别研究”(21701203);周口师范学院校本项目“DNA碱基切除修复蛋白超灵敏检测的电化学传感新方法研究”(ZKNUB1201701)。

摘　　要：多核苷酸激酶(PNK)在许多重要的生物进程的调节中起着关键作用,一些严重的疾病与PNK活性密切相关,发展高灵敏的PNK活性检测方法具有重要的意义.以T4 PNK为例,对近年来发展的PNK定量检测方法进行综述,主要介绍了荧光分析法和电化学法,并对PNK检测方法未来的发展前景进行了展望.Polynucleotide kinase(PNK)plays a key role in regulation of many important biological processes,and some serious diseases are closely related to PNK activity.Therefore,it is of great significance for the development of highly sensitive methods for detection of PNK activity.In order to overcome the shortcomings of traditional methods,modern detection methods for PNK have developed rapidly.Taking T4 PNK as an example,this paper reviews the quantitative detection methods for PNK developed in recent years,mainly introduces the fluorescence analysis methods and electrochemical methods,and the development prospect of PNK detection method in the future is prospected.

关 键 词：多核苷酸激酶 荧光分析法 电化学法 定量检测 DNA磷酸化 

分 类 号：O652[理学—分析化学]